DETECTION OF TRICHOMONAS VAGINALIS ANTIGEN BY DOT-ENZYME LINKED IMMUNOSORBENT ASSAY

A Dot-enzyme linked immunosorbent assay (Dot-ELISA) was developed for the detection of Trichomonas vaginalis antigen and compared for sensitivity,specificity and positive retes with wetfilm examination and culture. Of the specimens form 101 women testeds, 14 (13. 9 % ) were positive for T vaginalis by Dot-ELISA, 11 (10. 9% ) by wet film, 12 (11- 9 % ) by culture. Taken culture as the reference method, the Dot-ELISA had a sensitivity of 91. 7% and a specificity of 96. 0 %.