Objective To analyze the chemical constituents in the fermentation broth of the symbiotic fungus Myceliophthora lutea (M.lutea) ML-1 derived from Concha ostreae through liquid chromatography tandem mass spectrometry (LC-MS/MS)-based molecular networking so as to discover the secondary metabolite profile of this strain.
Methods M.lutea ML-1 was cultured in fungal medium number 1. The LC-MS/MS raw data was converted to. mzXML file by MSconvert software, and then uploaded to Global Natural Products Social Molecular Networking (GNPS) platform to establish the classical molecular networking (CMN) for identification of the chemical constituents and prediction of structure. Moreover, LC-MS/MS raw data were pre-processed by MZmine 2 software, and also uploaded to GNPS to create feature-based molecular networking (FBMN). Finally, CMN and FBMN were imported into Cytoscape software for data visualization.
Results According to CMN, a total of 49 compounds were identified from the fermentation broth of M.lutea ML-1, including 18 fatty acids, 8 anthraquinones, 12 cyclic dipeptides and 11 other compounds, in which there might be two new anthraquinone compounds. The relative quantitative analysis of chemical composition of fermentation broth based on FBMN had identified 3 compounds.
Conclusion The fermentation broth of M.lutea ML-1 cultured in fungal medium number 1 is rich in chemical constituents, which contains many highly potential active compounds. The results provide ideas for guided isolation of the chemical constituents and optimization of fermentation conditions for the strain in the future.
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