Objective To explore the mechanisms of Marsdenia tenacissima in inhibiting gastric cancer based on network pharmacology and molecular docking technology.
Methods PubMed database was used to search the literatures, and main active ingredients of Marsdenia tenacissima were screened by Chemical Composition Database and Swiss ADME. The core targets related to Marsdenia tenacissima were screened by retrieving disease databases, and protein-protein interaction (PPI) network map based on common target of active ingredients and gastric cancer was constructed by network pharmacology. AutoDock and PyMOL were used to perform molecular docking verification of active ingredients with key targets. DAVID database was used to perform enrichment analysis of key proteins. The molecular mechanism of Marsdenia tenacissima in inhibiting gastric cancer was predicted by their combined results.
Results Marsdenia tenacissima in inhibiting gastric cancer had thirty-six effective active components, acting on 116 target proteins. It was predicted that the pathways were related ErbB, VEGF, EGFR, PI3K/Akt, etc.
Conclusion Marsdenia tenacissima inhibits the invasion and metastasis of gastric cancer through the regulation of the key proteins such as SRC, PTK2 and HSP90AA1. The molecular mechanisms may be associated with PI3K/Akt, Ras/Raf/MEK/ERK or SRC/FAK signaling pathways, thereby treating gastric cancer.
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