Objective To investigate effect of down-regulating lncRNA HOXA-AS3 targeted promotion of miR-29b on the apoptosis of colon cancer cells LOVO and the sensitivity of oxaliplatin.
Methods Real-time polymerase chain reaction was used to detect the changes of HOXA-AS3 expression in colon cancer cells LOVO, SW1116, HCT116, SW620 and normal colon epithelial cells NCM460. Colon cancer cells LOVO were divided into control group, si-NC group, si-HOXA-AS3 group, L-OHP+si-NC group, L-OHP+si-HOXA-AS3 group, si-HOXA-AS3 +Anti-miR-NC group, si-HOXA-AS3 +Anti-miR-29b group, L-OHP+si-HOXA-AS3 +Anti-miR-NC group and L-OHP+si-HOXA-AS3 +Anti-miR-29b group. MTT assay, flow cytometry and Western blot were used to analyze cell proliferation, apoptosis and protein expression of Bax and Bcl-2, respectively. The bioinformatics software predicted the target miRNA of HOXA-AS3, and the luciferase activity assay kit was used to verify the targeting relationship between HOXA-AS3 and miR-29b.
Results Compared with normal colonic epithelial cells NCM460, the expression of HOXA-AS3 in colon cancer cells LOVO, SW1116, HCT116 and SW620 were significantly higher (P < 0.05). The expression level of HOXA-AS3 in colon cancer cell LOVO was significantly higher than colon cancer cell SW1116, HCT116 and SW620 (P < 0.05). Compared with the control and si-NC groups, the si-HOXA-AS3 colon cancer cell LOVO proliferation activity decreased, the apoptosis rate increased, the Bax protein expression level in the cells increased, and the Bcl-2 protein expression decreased (P < 0.05). Compared with the si-HOXA-AS3 and L-OHP+si-NC groups, the colon cancer cell LOVO proliferation activity in the L-OHP+si-HOXA-AS3 group decreased, the apoptosis rate increased, and the expression level of Bax protein in the cells increased, the expression of Bcl-2 protein decreased (P < 0.05). Compared with the si-HOXA-AS3 +Anti-miR-NC group, the si-HOXA-AS3 +Anti-miR-29b colon cancer cell LOVO proliferation activity increased, the apoptosis rate decreased, the Bax protein expression in the cells decreased, and Bcl-2 protein expression increased (P < 0.05). Compared with the L-OHP+si-HOXA-AS3 +Anti-miR-NC group, the colon cancer cell LOVO proliferation activity in the L-OHP+si-HOXA-AS3 +Anti-miR-29b group increased, and the apoptosis rate decreased, the expression of Bax protein in cells decreased, and the expression of Bcl-2 protein increased (P < 0.05). Down-regulation of HOXA-AS3 targeted to promote miR-29b expression.
Conclusion Down-regulation of HOXA-AS3 targeting promotes miR-29b to induce the apoptosis of colon cancer cells LOVO and increase the sensitivity of oxaliplatin.
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