Correlations between TPMT and NUDT15 gene polymorphisms and intestinal wall inflammatory response as well as energy spectrum imaging in the patients with inflammatory bowel disease

  Objective  To investigate the correlations between thiopurine methyltransferase (TPMT) and nucleoside diphophate-linked moiety X-type motif 15 (NUDT15) gene polymorphisms and inflammatory response of the intestinal wall as well as energy spectrum imaging in patients with inflammatory bowel disease (IBD).

  Methods  Peripheral blood samples of 60 Chinese patients with IBD and 60 healthy people were obtained and their DNA was extracted. Genomic DNA was used as the template for polymerase chain reaction (PCR), genotyping was performed on the variant of R139C NUDT15 gene and TPMT variant of TPMT*3C. The correlations between TPMT, NUDT15 gene polymorphisms and inflammatory response of the intestinal wall after azathioprine (AZA) treatment as well as energy spectrum imaging were analyzed.

  Results  In TPMT c.719A>G, the frequencies of AA genotype and A allele decreased significantly in the IBD group (P < 0.05), and the frequencies of AG and GG genotype and G allele increased significantly (P < 0.05). In NUDT15 c.415C>T, the CC genotype and C allele frequencies decreased significantly in the IBD group (P < 0.05), and the CT and TT genotypes and T allele frequencies increased significantly in the IBD group (P < 0.05). The remission rates of intestinal wall inflammatory response in TPMT AA genotype and A allele frequencies increased significantly when compared with AG and GG genotype and G allele frequencies (P < 0.05), while those in NUDT15 CC genotype and C allele frequencies increased significantly when compared with CT and TT genotype and T allele frequencies (P < 0.05). The detection rates of CT energy spectrum imaging in TPMT AA genotype and A allele frequencies were significantly lower than that in AG and GG genotype and G allele frequencies (P < 0.05). The detection rates of energy spectrum imaging in NUDT15 CC genotype and C allele frequencies were significantly lower than those in CT and TT genotype and T alleles frequencies (P < 0.05).

  Conclusion  The mutation rate of NUDT15 in Chinese IBD patients is significantly higher than that of TPMT. NUDT15 polymorphism is more predictable than TPMT polymorphism in AZA-induced intestinal wall inflammatory response and detection rate of energy spectrum imaging.