GKT137831 alleviates atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level

  Objective  To explore the mechanism of GKT137831 on protecting skeletal muscle cells by atorvastatin intervention.

  Methods  Atorvastatin was used to intervene C2C12 cells in skeletal muscle for 48 hours. CCK-8 method was used to analyze the effect of atorvastatin on survival rate of skeletal muscle cells. Western blot was used to detect the expression of NADPH Oxidase 4 (Nox4) after atorvastatin intervention. Flow cytometry was used to detect the effect of atorvastatin on glucose uptake and reactive oxygen species (ROS) in skeletal muscle cells. Co-culture of Nox4 inhibitor GKT137831 was used to analyze the effect of atorvastatin on cellular glucose uptake and ROS level.

  Results  After intervention with atorvastatin on skeletal muscle cells for 48 hours, the glucose uptake ability of C2C12 cells decreased and ROS increased. GKT137831 could alleviate the decrease of glucose uptake and the increase of ROS. CCK-8 results showed that atorvastatin had no effect on cell viability after 48 hours of intervention. Nox4 expression increased after atorvastatin intervention on C2C12 skeletal muscle cells.

  Conclusion  GKT137831 can alleviate atorvastatin-induced decreased glucose uptake of skeletal muscle by suppressing reactive oxygen species level.