Abstract: Objective To explore the correlation between the prevalence of thyroid nodule and the metabolism of blood glucose as well as lipid and analyze the risk factors affecting the prevalence of thyroid nodule. Methods From January to December 2018, the materials of 3 840 healthy people with thyroid function(5 items)test and thyroid ultrasound in the physical examination center of Shengjing Hospital Affiliated to China Medical University were collected. According to the fasting blood glucose level, the subjects were divided into normal blood glucose group, increased blood glucose group and diabetes group. According to incidence of thyroid nodule, the subjects were divided into thyroid nodule positive group and thyroid nodule negative group. The prevalence of thyroid nodule and related biochemical indexes were analyzed in all the groups. Results A total of 1 942 patients were confirmed as thyroid nodules in this study, and the prevalence of thyroid nodules was 50.57%. The prevalence of thyroid nodules in men was 46.83%, while was 55.89% in women, and prevalence of thyroid nodules in women was significantly higher than that in men(P<0.05). With the increase of age, the prevalence of thyroid nodules increased significantly(P<0.05). The levels of fasting blood glucose, total cholesterol and low density lipoprotein cholesterol in the thyroid nodule positive group were significantly higher than those in the thyroid nodule negative group(P<0.01). The prevalence of thyroid nodule increased with the increase of blood glucose level. The prevalence of thyroid nodule - in normal blood glucose group was 49.18%, which was significantly lower than 56.13% in increased blood glucose group and 58.64% in diabetes group(P<0.05 or P<0.01). Multiple Logistic regression analysis showed that gender and age were the independent influencing factors for thyroid nodules in healthy people in Shenyang area. Conclusion The prevalence of thyroid nodule is higher in people with physical examinations in our hospital. Gender and age are the independent influencing factors for thyroid nodule, and the abnormal blood glucose and lipid may also lead to the increased prevalence of thyroid nodule, which needs to be paid attention.