| Citation: |
WEI Man, YUAN Wenhe, LIU Hongyun, MA Dimeng, YAN Kanglu, HAN Ning. Expression levels of serum secreted frizzled-related protein 5, heat shock protein 60 and solute carrier family 16 member 11 in patients with gestational diabetes mellitus and their relationships with insulin resistance[J]. Journal of Clinical Medicine in Practice, 2024, 28(20): 60-65. DOI: 10.7619/jcmp.20242397
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To investigate the expression levels of secreted frizzled-related protein 5 (sFRP5), heat shock protein 60 (HSP60) and solute carrier family 16 member 11 (SLC16A11) in serum of patients with gestational diabetes mellitus (GDM) and their relationships with insulin resistance.
A total of 120 GDM patients in the hospital from January 2022 to December 2023 were selected as study group, and another 120 healthy pregnant women in the same period were selected as control group. The expression levels of serum sFRP5, HSP60 and SLC16A11 were detected; the levels of insulin resistance-related indicators[fasting blood glucose (FBG), fasting insulin (FINS), and homeostasis model assessment of insulin resistance (HOMA-IR)]were measured and calculated; the Pearson's correlation method was used to analyze the correlations of serum sFRP5, HSP60 and SLC16A11 with insulin resistance; the Logistic regression andysis and receiver operating characteristic (ROC) curve analysis were used to assess the relationships of serum sFRP5, HSP60 and SLC16A11 with GDM.
The serum sFRP5 expression in the study group was significantly lower than that in the control group, while the serum HSP60, SLC16A11, FBG, FINS and HOMA-IR levels were significantly higher than those in the control group (P < 0.05). Pearson's correlation analysis showed that serum sFRP5 was negatively correlated with HSP60, SLC16A11, FBG, FINS and HOMA-IR (P < 0.05); serum HSP60 was positively correlated with SLC16A11, FBG, FINS and HOMA-IR (P < 0.05); serum SLC16A11 was positively correlated with FBG, FINS and HOMA-IR (P < 0.05). Multivariate Logistic regression analysis revealed that HSP60, SLC16A11, FBG, FINS and HOMA-IR were risk factors for GDM in pregnant patients, while sFRP5 was a protective factor (P < 0.05). The area under the curve (AUC) for the combined diagnosis of GDM in pregnant patients by serum sFRP5, HSP60 and SLC16A11 was 0.924, indicating that the combined diagnostic value of three indicators was superior to that of each individual indicator (all P < 0.05).
GDM patients exhibit decreased serum sFRP5 level and increased HSP60 and SLC16A11 levels; three indicators are factors influencing the occurrence of GDM in pregnant patients, and are associated with insulin resistance; the combination of three indicators exhibits high diagnostic efficacy for GDM.
| [1] |
YE W R, LUO C, HUANG J, et al. Gestational diabetes mellitus and adverse pregnancy outcomes: systematic review and meta-analysis[J]. BMJ, 2022, 377: e067946.
|
| [2] |
HILDÉN K, HANSON U, PERSSON M, et al. Gestational diabetes and adiposity are independent risk factors for perinatal outcomes: a population based cohort study in Sweden[J]. Diabet Med, 2019, 36(2): 151-157. doi: 10.1111/dme.13843
|
| [3] |
ZHANG Y H, CHEN Y Y, QU H M, et al. Methylation of HIF3A promoter CpG islands contributes to insulin resistance in gestational diabetes mellitus[J]. Mol Genet Genomic Med, 2019, 7(4): e00583. doi: 10.1002/mgg3.583
|
| [4] |
SERT U Y, OZGU-ERDINC A S. Gestational diabetes mellitus screening and diagnosis[J]. Adv Exp Med Biol, 2021, 1307: 231-255.
|
| [5] |
LI Y, TIAN M Y, YANG M L, et al. Central Sfrp5 regulates hepatic glucose flux and VLDL-triglyceride secretion[J]. Metabolism, 2020, 103: 154029. doi: 10.1016/j.metabol.2019.154029
|
| [6] |
朱伟, 郑世霞, 柯琳秋, 等. SFRP5对小鼠肝癌细胞系Hepa1-6糖代谢的影响[J]. 基础医学与临床, 2018, 38(9): 1268-1273.
|
| [7] |
ZHANG D L, LIU H, ZHANG Y M, et al. Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor[J]. Cell Commun Signal, 2020, 18(1): 60. doi: 10.1186/s12964-020-00546-5
|
| [8] |
WU X X, GUO J Y, CHEN Y L, et al. The 60-kDa heat shock protein regulates energy rearrangement and protein synthesis to promote proliferation of multiple myeloma cells[J]. Br J Haematol, 2020, 190(5): 741-752. doi: 10.1111/bjh.16569
|
| [9] |
HIDALGO B A, SOFER T, QI Q B, et al. Associations between SLC16A11 variants and diabetes in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)[J]. Sci Rep, 2019, 9(1): 843. doi: 10.1038/s41598-018-35707-7
|
| [10] |
中华医学会妇产科学分会产科学组, 中华医学会围产医学分会妊娠合并糖尿病协作组. 妊娠合并糖尿病诊治指南(2014)[J]. 中华妇产科杂志, 2014, 49(8): 561-569.
|
| [11] |
XIE J W, LI L, XING H Y. Metabolomics in gestational diabetes mellitus: a review[J]. Clin Chim Acta, 2023, 539: 134-143. doi: 10.1016/j.cca.2022.12.005
|
| [12] |
DŁUSKI D F, WOLINSKA E, SKRZYPCZAK M. Epigenetic changes in gestational diabetes mellitus[J]. Int J Mol Sci, 2021, 22(14): 7649. doi: 10.3390/ijms22147649
|
| [13] |
JOHNS E C, DENISON F C, NORMAN J E, et al. Gestationaldiabetes mellitus: mechanisms, treatment, and complications[J]. Trends Endocrinol Metab, 2018, 29(11): 743-754. doi: 10.1016/j.tem.2018.09.004
|
| [14] |
王志强, 吴剑华, 庄伟, 等. 利拉鲁肽对耐克罗米芬多囊卵巢综合征患者氧化应激, 胰岛素抵抗及血清SFRP5, IL-34水平的影响[J]. 中国妇产科临床杂志, 2023, 24(3): 307-309.
|
| [15] |
姜瑞丰, 施妙君, 鄢巨振. 维持性血液透析患者血清Shp5、Klotho水平变化及其与钙磷代谢的关系[J]. 广东医学, 2020, 41(2): 152-155.
|
| [16] |
刘敏, 缪华珍. GDM患者miRNA-125b、SFRP5、SerpinBl变化及益生菌联合胰岛素治疗效果[J]. 中国计划生育杂志, 2023, 31(10): 2354-2358.
|
| [17] |
方云艳, 秦雯, 王小蓓, 等. 血清sFRP5与老年2型糖尿病患者视网膜病变的相关性[J]. 国际检验医学杂志, 2023, 44(21): 2602-2606.
|
| [18] |
刘莉芳, 赵桁, 张云良, 等. 2型糖尿病患者血清Nesfatin-1、HSP60与下肢血管病变的相关性研究[J]. 中国动脉硬化杂志, 2021, 29(11): 965-970.
|
| [19] |
马聆桦, 李江恒, 钟柳育, 等. 高龄孕产妇临床特征及孕检对妊娠风险的影响[J]. 实用临床医药杂志, 2022, 26(9): 50-53. doi: 10.7619/jcmp.20214722
|
| [20] |
TIMOFEEV Y S, KISELEV A R, DZHIOEVA O N, et al. Heat shock proteins (HSPs) and cardiovascular complications of obesity: searching for potential biomarkers[J]. Curr Issues Mol Biol, 2023, 45(12): 9378-9389. doi: 10.3390/cimb45120588
|
| [21] |
ALUKSANASUWAN S, SUEKSAKIT K, FONG-NGERN K, et al. Role of HSP60 (HSPD1) in diabetes-induced renal tubular dysfunction: regulation of intracellular protein aggregation, ATP production, and oxidative stress[J]. FASEB J, 2017, 31(5): 2157-2167. doi: 10.1096/fj.201600910RR
|
| [22] |
ZIMBONE S, DI ROSA M C, CHIECHIO S, et al. Exploring the role of Hsp60 in Alzheimer's disease and type 2 diabetes: suggestion for common drug targeting[J]. Int J Mol Sci, 2023, 24(15): 12456. doi: 10.3390/ijms241512456
|
| [23] |
张坦, 漆正堂, 丁树哲. SLC16A11调控小鼠骨骼肌Akt/GLUT4通路的研究及运动对SLC16A11和GLUT4表达的影响[J]. 中国运动医学杂志, 2022, 41(1): 43-51.
|
| [24] |
THILAK S, RAJENDRA A, GANESH V. Association of obesity and insulin resistance to gestational diabetes mellitus[J]. Bioinformation, 2023, 19(2): 211-214. doi: 10.6026/97320630019211
|
| [25] |
陈辰, 陈亚军, 戴楠, 等. 妊娠期糖尿病患者孕早期糖脂代谢状态的研究[J]. 中国妇幼保健, 2018, 33(15): 3445-3447.
|
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