Objective To investigate the effects of isoproterenol administration on myocardial injury and establish acute myocardial infarction (AMI) model in mice.
Methods Mice were injected subcutaneously with isoproterenol (150 mg/kg), and blood samples were taken at different time points. The contents of AMI related indexes creatine kinase (CK), creatine kinase isoenzyme (CK-MB), cardiac troponin T (cTnT), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL-6), procalcitonin (PCT) and C-reactive protein (CRP) in serum were detected. The pathological changes of cardiac tissue were observed by hematoxylin-eosin staining.
Results The serum levels of CK, CK-MB, LDH, cTnT, ALT and AST were significantly increased after isoproterenol model (P < 0.05, P < 0.01); after two consecutive administrations of isoproterenol, the serum AMI-related indexes increased significantly, and the relevant indexes reached the highest concentration 16 h after administration (P < 0.05, P < 0.01). Hematoxylin-eosin staining results showed that pathological changes occurred in myocardial tissue.
Conclusion Subcutaneous injection of isoproterenol induces AMI injury. The AMI injury at 16 h after two consecutive injections is the most serious, which lays a foundation for clinical drug research and innovative drug development of AMI.
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