Mechanism of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and network pharmacology

PANG Hanqing; SHANG Xiaoyu; WEI Ye; DING Shuwen; LIU Liang

doi:10.7619/jcmp.20223482

Journal of Clinical Medicine in Practice > 2022 > 26(24): 13-19. > DOI: 10.7619/jcmp.20223482

PANG Hanqing, SHANG Xiaoyu, WEI Ye, DING Shuwen, LIU Liang. Mechanism of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and network pharmacology[J]. Journal of Clinical Medicine in Practice, 2022, 26(24): 13-19. DOI: 10.7619/jcmp.20223482

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Mechanism of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and network pharmacology

PANG Hanqing^{1, 2},
SHANG Xiaoyu¹,
WEI Ye¹,
DING Shuwen¹,
LIU Liang^{1, 2, ,}

1.
Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001
2.
Jiangsu KeyLaboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou, Jiangsu, 225000

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Received Date: November 20, 2022
Available Online: January 06, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore the mechanisms of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) and network pharmacology technology.
Methods
The chemical compounds of Tongmai granules were identified by UHPLC-QTOF-MS approach. The compounds' targets and stroke-related targets were screened via TCMSP, TTD and PubMed databases. STRING database was used to construct the protein interaction network of key targets. DAVID database was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis for key targets.
Results
A total of 96 chemical components, such as salvianolic acid B, puerarin and ligustilide were identifiedfrom Tongmai granules. A total of 38 active compounds and 55 targets of Tongmai granules for stroke treatment were obtained. The core targets included prostaglandin-endoperoxide synthase 2 (PTGS2), nuclear factor kappa B (NFκB)as well as nuclear factor erythroid 2-related factor 2 (NRF2) and so on, and the key pathways mainly involved hypoxia-inducible factor-1 (HIF-1), tumor necrosis factor (TNF) and phosphatidylinositol 3-kinase (PI3K)-Akt and so on.
Conclusion
Major active components such as salvianolic acid B, puerarin and ligustilide in Tongmai granules regulate HIF-1, TNF and PI3K-Akt signaling pathways by acting on PTGS2, NFκB and NRF2 and other targets to play an anti-stroke role.

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References (27)

References

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Mechanism of Tongmai granules in treatment of stroke based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry and network pharmacology

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