Mechanism of long non-coding RNA NR2F1-AS1 regulates proliferation and apoptosis of ovarian cancer cells by targeting microRNA-493-5p expression

ZHANG Yu; ZHANG Can; ZHANG Weiling

doi:10.7619/jcmp.20222967

Journal of Clinical Medicine in Practice > 2023 > 27(4): 30-34. > DOI: 10.7619/jcmp.20222967

ZHANG Yu, ZHANG Can, ZHANG Weiling. Mechanism of long non-coding RNA NR2F1-AS1 regulates proliferation and apoptosis of ovarian cancer cells by targeting microRNA-493-5p expression[J]. Journal of Clinical Medicine in Practice, 2023, 27(4): 30-34. DOI: 10.7619/jcmp.20222967

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Mechanism of long non-coding RNA NR2F1-AS1 regulates proliferation and apoptosis of ovarian cancer cells by targeting microRNA-493-5p expression

Department of Gynecology, Nantong City Cancer Hospital in Jiangsu Province, Nantong, Jiangsu, 226361

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Received Date: September 21, 2022
Available Online: March 14, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore the mechanism of long non-coding RNA NR2F1-AS1 (LncRNA NR2F1-AS1) in regulating proliferation and apoptosis of ovarian cancer cells by targeting microRNA-493-5p (miR-493-5p) expression.
Methods
The quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of NR2F1-AS1 and miR-493-5p in different lines of ovarian cancer cells; the dual luciferase reporter gene was used to detect the interaction between NR2F1-AS1 and miR-493-5p; the MTT proliferation assay was used to determine the change in the proliferation ability of ovarian cancer cells after interference with NR2F1-AS1 expression or miR-493-5p overexpression; the apoptosis rate of ovarian cancer cells was detected by flow cytometry; the Western blot was used to detect the protein expression levels of CyclinD1, p21, p27, Bcl-2, Bax and cleaved-caspase 3.
Results
Compared with normal ovarian epithelial cells, the expression level of NR2F1-AS1 in ovarian cancer cells increased significantly, while the expression level of miR-493-5p decreased significantly (P < 0.05). The dual luciferase assay confirmed that NR2F1-AS1 could specifically bind to miR-493-5p, and the expression and activity of miR-493-5p were negatively regulated by NR2F1-AS1. Interference with NR2F1-AS1 expression or overexpression of miR-493-5p could inhibit the proliferation of ovarian cancer cells, promote apoptosis, significantly up-regulate the expression of p21, Bax, p27 and cleaved-caspase 3, and down-regulate protein expression levels of CyclinD1 and Bcl-2. Inhibiting the expression of miR-493-5p was able to reverse the effect of interference with the expression of NR2F1-AS1 on the proliferation and apoptosis of ovarian cancer cells.
Conclusion
Interference with the expression of NR2F1-AS1 can inhibit the proliferation of ovarian cancer cells and induce apoptosis by up-regulating the expression of miR-493-5p.
- ovarian cancer,
- long non-coding RNA NR2F1-AS1,
- microRNA-493-5p,
- proliferation,
- apoptosis

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References (13)

References

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Mechanism of long non-coding RNA NR2F1-AS1 regulates proliferation and apoptosis of ovarian cancer cells by targeting microRNA-493-5p expression