Mechanism of LncRNA XIST regulating proliferation, migration, invasion and apoptosis of breast cancer cells through miR-20a-5p/HMGA2 axis

GUO Chenming; LUO Zhiwen; KONG Han; YANG Zhen; WANG Yiyang; Dilimulati·AISIMUTULA

doi:10.7619/jcmp.20222508

Journal of Clinical Medicine in Practice > 2023 > 27(5): 61-66, 71. > DOI: 10.7619/jcmp.20222508

GUO Chenming, LUO Zhiwen, KONG Han, YANG Zhen, WANG Yiyang, Dilimulati·AISIMUTULA. Mechanism of LncRNA XIST regulating proliferation, migration, invasion and apoptosis of breast cancer cells through miR-20a-5p/HMGA2 axis[J]. Journal of Clinical Medicine in Practice, 2023, 27(5): 61-66, 71. DOI: 10.7619/jcmp.20222508

Citation:

PDF (4706 KB)

Mechanism of LncRNA XIST regulating proliferation, migration, invasion and apoptosis of breast cancer cells through miR-20a-5p/HMGA2 axis

Department of Breast Surgery of Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 930054

More Information

Received Date: August 14, 2022
Revised Date: September 08, 2022
Available Online: April 06, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the effects and mechanisms of LncRNA XIST on proliferation, migration, invasion and apoptosis of breast cancer cells.
Methods
Normal mammary epithelial cells MCF-10A and breast cancer cell lines MCF-7, T47D and Bcap-37 cells were selected and transfected into MCF-7 and T47D cells with different substances. They were set as blank group (no transfection), si-XIST group (transfection si-XIST), si-NC group (transfection si-NC), microRNA-20a-5p group (transfection miR-20a-5p mimics) and miR-NC group (transfection miR-NC). CCK-8 was used to detect cell proliferation; Transwell was used to detect cell invasion ability; cell migration ability was detected by scratch test; apoptosis was detected by TUNEL staining. Luciferase reporter gene assay was used to verify the targeting relationships between LncRNA XIST and miR-20a-5p as well as between miR-20a-5p and high mobility group protein A2 (HMGA2). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of LncRNA XIST and miR-20a-5p. The expression level of HMGA2 was detected by Western blot.
Results
Compared with MCF-10A cells, LncRNA XIST expression levels in MCF-7, T47D and Bcap-37 cells were significantly increased, while miR-20a-5p expression level was significantly decreased (P < 0.05). Down-regulating LncRNA XIST or up-regulating miR-20a-5p inhibited proliferation, invasion and migration of breast cancer cells, and promoted cell apoptosis (P < 0.05). LncRNA XIST negatively regulated miR-20a-5p, and miR-20a-5p negatively regulated HMGA2.
Conclusion
Down-regulated LncRNA XIST inhibits proliferation, migration andinvasion of breast cancer cells and promotes cell apoptosis through miR-20a-5p/HMGA2 axis.
- breast cancer,
- long non-coding RNA XIST,
- microRNA-20a-5p,
- high mobility group protein A2

FullText(HTML)

References (20)

References

[1]	KATSURA C, OGUNMWONYI I, KANKAM H K, et al. Breast cancer: presentation, investigation and management[J]. Br J Hosp Med (Lond), 2022, 83(2): 1-7.
[2]	WINKLE M, EL-DALY S M, FABBRI M, et al. Noncoding RNA therapeutics-challenges and potential solutions[J]. Nat Rev Drug Discov, 2021, 20(8): 629-651. doi: 10.1038/s41573-021-00219-z
[3]	SMOLARZ B, ZADROZNA-NOWAK A, ROMANOWICZ H. The role of LncRNA in the development of tumors, including breast cancer[J]. Int J Mol Sci, 2021, 22(16): 8427. doi: 10.3390/ijms22168427
[4]	LIU Y J, ZHANG P Y, WU Q Y, et al. Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63[J]. Nat Commun, 2021, 12(1): 5232. doi: 10.1038/s41467-021-25552-0
[5]	张娜, 易茂林. LncRNA XIST通过海绵吸附miR-133a调控甲状腺癌细胞的增殖和凋亡[J]. 贵州医科大学学报, 2021, 46(8): 917-924. https://www.cnki.com.cn/Article/CJFDTOTAL-GYYB202108008.htm
[6]	才昊, 韩亮, 李晓飞. LncRNA XIST通过调控miR-186-5p促进非小细胞肺癌的增殖和侵袭实验研究[J]. 陕西医学杂志, 2020, 49(7): 781-787, 803. doi: 10.3969/j.issn.1000-7377.2020.07.004
[7]	YAN Z Z, LI J, GUO J, et al. LncRNA XIST sponges microRNA-448 to promote malignant behaviors of colorectal cancer cells via regulating GRHL2[J]. Funct Integr Genomics, 2022, 22(5): 977-988. doi: 10.1007/s10142-022-00873-5
[8]	胡炜, 邵安静, 李春霞, 等. 沉默miR-20a-5p的肝癌细胞恶性生物学行为及Zbed3、Axin蛋白表达情况观察[J]. 山东医药, 2022, 62(11): 28-32. https://www.cnki.com.cn/Article/CJFDTOTAL-SDYY202211007.htm
[9]	LI J, YE D L, SHEN P, et al. MiR-20a-5p induced WTX deficiency promotes gastric cancer progressions through regulating PI3K/AKT signaling pathway[J]. J Exp Clin Cancer Res, 2020, 39(1): 212. doi: 10.1186/s13046-020-01718-4
[10]	XU J X, FANG X J, LONG L Y, et al. HMGA2 promotes breast cancer metastasis by modulating Hippo-YAP signaling pathway[J]. Cancer Biol Ther, 2021, 22(1): 5-11. doi: 10.1080/15384047.2020.1832429
[11]	ZHAO W Y, GENG D H, LI S Q, et al. LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer[J]. Cancer Med, 2018, 7(3): 842-855. doi: 10.1002/cam4.1353
[12]	LI P, WANG L H, LI P F, et al. Silencing lncRNA XIST exhibits antiproliferative and proapoptotic effects on gastric cancer cells by up-regulating microRNA-132 and down-regulating PXN[J]. Aging (Albany NY), 2020, 13(10): 14469-14481.
[13]	WANG H R, LI H M, YU Y K, et al. Long non-coding RNA XIST promotes the progression of esophageal squamous cell carcinoma through sponging miR-129-5p and upregulating CCND1 expression[J]. Cell Cycle, 2021, 20(1): 39-53. doi: 10.1080/15384101.2020.1856497
[14]	ZHANG M, WANG F, XIANG Z, et al. LncRNA XIST promotes chemoresistance of breast cancer cells to doxorubicin by sponging miR-200c-3p to upregulate ANLN[J]. Clin Exp Pharmacol Physiol, 2020, 47(8): 1464-1472. doi: 10.1111/1440-1681.13307
[15]	郭会, 方艳君, 李翠娥, 等. 过/降表达miR-20a-5p的骨肉瘤细胞增殖、侵袭能力及Bax、Bcl-2、E2F5蛋白表达观察[J]. 山东医药, 2022, 62(11): 42-46. https://www.cnki.com.cn/Article/CJFDTOTAL-SDYY202211010.htm
[16]	HE Y, MA H, WANG J, et al. MiR-20a-5p inhibits endometrial cancer progression by targeting Janus kinase 1[J]. Oncol Lett, 2021, 21(5): 427. doi: 10.3892/ol.2021.12688
[17]	YANG H B, CHEN Z, LIU Z M. MiR-20a-5p negatively regulates NR4A3 to promote metastasis in bladder cancer[J]. J Oncol, 2021, 2021: 1377989.
[18]	BAI X D, HAN G H, LIU Y, et al. MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3[J]. Biomed Pharmacother, 2018, 103: 1482-1489. doi: 10.1016/j.biopha.2018.04.165
[19]	李会芳, 侯永强, 王明军, 等. 乳腺癌组织FOXP3、HMGA2、RASAL2的表达及与临床病理特征和预后的关系[J]. 现代生物医学进展, 2021, 21(20): 3959-3964. https://www.cnki.com.cn/Article/CJFDTOTAL-SWCX202120032.htm
[20]	李晨阳, 张学红. MiR-154靶向HMGA2基因在人子宫内膜癌中的表达及其对癌细胞恶性生物学行为的影响[J]. 兰州大学学报: 医学版, 2022, 48(1): 21-27. https://www.cnki.com.cn/Article/CJFDTOTAL-LZYX202201006.htm

Cited By

Get Citation

PDF

XML

Article views (171) PDF downloads (16)

Turn off MathJax

Article Contents

Abstract

References

Mechanism of LncRNA XIST regulating proliferation, migration, invasion and apoptosis of breast cancer cells through miR-20a-5p/HMGA2 axis

Abstract

References

Catalog

Export File

Citation

Format

Content