ZHU Yaqian, HU Gaochao, QU Xingguang. Expression of serum inflammatory cytokines in patients with diabetic cardiomyopathy and their effects on mitochondrial function of cardiomyocytes[J]. Journal of Clinical Medicine in Practice, 2022, 26(24): 57-60, 85. DOI: 10.7619/jcmp.20222221
Citation: ZHU Yaqian, HU Gaochao, QU Xingguang. Expression of serum inflammatory cytokines in patients with diabetic cardiomyopathy and their effects on mitochondrial function of cardiomyocytes[J]. Journal of Clinical Medicine in Practice, 2022, 26(24): 57-60, 85. DOI: 10.7619/jcmp.20222221

Expression of serum inflammatory cytokines in patients with diabetic cardiomyopathy and their effects on mitochondrial function of cardiomyocytes

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  • Received Date: July 19, 2022
  • Available Online: January 06, 2023
  • Objective 

    To investigate the expression levels of inflammatory cytokines interleukin (IL)-1β and IL-18 in diabetic cardiomyopathy (DCM) and their effects on mitochondrial function of cardiomyocytes.

    Methods 

    The peripheral blood serum of 104 DCM patients (DCM group) and 90 healthy volunteers (healthy control group) were collected, and the serum levels of IL-1β and IL-18 in the two groups were detected. Human myocardial cell line AC16 was cultured and divided into control group, IL-1β group (adding 10 ng/mL recombinant human IL-1β) and IL-18 group (adding 10 ng/mL recombinant human IL-18). Cell proliferation activity was detected by CCK-8 assay; mitochondrial membrane potential and permeability were detected by JC-1 staining and calcein staining, respectively; the expression levels of apoptosis-related proteins Bax, Bcl-2 and Cleaved Caspase-3 (C-Casp3) were detected by western blot.

    Results 

    Compared with the healthy control group, serum IL-1β and IL-18 concentrations in the DCM group were increased, and the difference was statistically significant (P < 0.05). Compared with the control group, the proliferating activity of AC16 cells was significantly decreased after incubation with IL-1β or IL-18 for 48 and 72 h (P < 0.05); compared with the control group, the percentage of cells with decreased mitochondrial membrane potential in the IL-1β and IL-18 groups was significantly increased (P < 0.001); the average fluorescence intensity of calcein in the IL-1β group and IL-18 group was significantly higher than that in the control group (P < 0.001). Compared with the control group, the Bax/Bcl-2 and C-Casp3/GAPDH in the IL-1β and IL-18 groups were significantly increased (P < 0.01 or P < 0.001).

    Conclusion 

    The expression levels of IL-1β and IL-18 in peripheral blood of DCM patients are increased, and the function of cardiomyocytes is affected by inhibiting mitochondrial activity.

  • [1]
    MARFELLA R, SARDU C, MANSUETO G, et al. Evidence for human diabetic cardiomyopathy[J]. Acta Diabetol, 2021, 58(8): 983-988. doi: 10.1007/s00592-021-01705-x
    [2]
    SALVATORE T, PAFUNDI P C, GALIERO R, et al. The diabetic cardiomyopathy: the contributing pathophysiological mechanisms[J]. Front Med (Lausanne), 2021, 8: 695792.
    [3]
    ABDELRAHMAN A H, SALAMA I I, SALAMA S I, et al. Role of some serum biomarkers in the early detection of diabetic cardiomyopathy[J]. Future Sci OA, 2021, 7(5): FSO682. doi: 10.2144/fsoa-2020-0184
    [4]
    YOUSSEF M E, ABDELRAZEK H M, MOUSTAFA Y M. Cardioprotective role of GTS-21 by attenuating the TLR4/NF-κB pathway in streptozotocin-induced diabetic cardiomyopathy in rats[J]. Naunyn Schmiedebergs Arch Pharmacol, 2021, 394(1): 11-31. doi: 10.1007/s00210-020-01957-4
    [5]
    RATHINAM V A K, FITZGERALD K A. Inflammasome complexes: emerging mechanisms and effector functions[J]. Cell, 2016, 165(4): 792-800. doi: 10.1016/j.cell.2016.03.046
    [6]
    YANG F, QIN Y, WANG Y Q, et al. Metformin inhibits the NLRP3 inflammasome via AMPK/mTOR-dependent effects in diabetic cardiomyopathy[J]. Int J Biol Sci, 2019, 15(5): 1010-1019. doi: 10.7150/ijbs.29680
    [7]
    SONG S, DING Y, DAI G L, et al. Sirtuin 3 deficiency exacerbates diabetic cardiomyopathy via necroptosis enhancement and NLRP3 activation[J]. Acta Pharmacol Sin, 2021, 42(2): 230-241. doi: 10.1038/s41401-020-0490-7
    [8]
    HE Y, HARA H, NUÑEZ G. Mechanism and regulation of NLRP3 inflammasome activation[J]. Trends Biochem Sci, 2016, 41(12): 1012-1021. doi: 10.1016/j.tibs.2016.09.002
    [9]
    ABBATE A, TOLDO S, MARCHETTI C, et al. Interleukin-1 and the inflammasome as therapeutic targets in cardiovascular disease[J]. Circ Res, 2020, 126(9): 1260-1280. doi: 10.1161/CIRCRESAHA.120.315937
    [10]
    LIBBY P. Targeting inflammatory pathways in cardiovascular disease: the inflammasome, interleukin-1, interleukin-6 and beyond[J]. Cells, 2021, 10(4): 951. doi: 10.3390/cells10040951
    [11]
    BUSCH K, KNY M, HUANG N, et al. Inhibition of the NLRP3/IL-1β axis protects against Sepsis-induced cardiomyopathy[J]. J Cachexia Sarcopenia Muscle, 2021, 12(6): 1653-1668. doi: 10.1002/jcsm.12763
    [12]
    LI Z H, HU S Q, HUANG K, et al. Targeted anti-IL-1β platelet microparticles for cardiac detoxing and repair[J]. Sci Adv, 2020, 6(6): eaay0589. doi: 10.1126/sciadv.aay0589
    [13]
    XIAO H, LI H, WANG J J, et al. IL-18 cleavage triggers cardiac inflammation and fibrosis upon β-adrenergic insult[J]. Eur Heart J, 2018, 39(1): 60-69. doi: 10.1093/eurheartj/ehx261
    [14]
    ABATE M, FESTA A, FALCO M, et al. Mitochondria as playmakers of apoptosis, autophagy and senescence[J]. Semin Cell Dev Biol, 2020, 98: 139-153. doi: 10.1016/j.semcdb.2019.05.022
    [15]
    DABRAVOLSKI S A, ZHURAVLEV A D, KARTUESOV A G, et al. Mitochondria-mediated cardiovascular benefits of sodium-glucose co-transporter 2 inhibitors[J]. Int J Mol Sci, 2022, 23(10): 5371. doi: 10.3390/ijms23105371
    [16]
    YANG F, PEI R N, ZHANG Z W, et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes[J]. Toxicol In Vitro, 2019, 54: 310-316. doi: 10.1016/j.tiv.2018.10.017
    [17]
    LIU S P, ZHU Y Y, YAN S S, et al. Phenethyl isothiocyanate induces IPEC-J2 cells cytotoxicity and apoptosis via S-G2/M phase arrest and mitochondria-mediated Bax/Bcl-2 pathway[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2019, 226: 108574. doi: 10.1016/j.cbpc.2019.108574

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