Objective To investigate the predictive value of serum microRNA-128-3p (miR-128-3p) for in-stent restenosis (ISR) after endovascular intervention in patients with lower extremity atherosclerotic occlusive disease (LEAOD).
Methods A total of 406 LEAOD patients undergoing endovascular interventional therapy were recruited as study objects.At 6 months of follow-up, patients were divided into restenosis group (n=79) and the non-restenosis group (n=327).The levels of serum miR-128-3p were detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR), and the levels of serum interleukin (IL)-6, IL-8 and metalloproteinase tissue inhibitor 1(TIMP-1) were detected by enzyme-linked immunosorbent assay.
Results The levels of serum miR-128-3p levels after surgery in both groups were lower than before surgery, and the serum miR-128-3p levels before and after surgery in the restenosis group were higher than those in the non-restenosis group, the differences were statistically significant (P < 0.05).Receiver operating characteristic curve analysis showed that area under curve of preoperative serum miR-128-3p level in predicting ISR was 0.712(95%CI, 0.644 to 0.781).The miR-128-3p levels were significantly higher in patients with diabetes, hypertension or age≥50 years in the restenosis group (P < 0.05).Age≥50 years, Fontaine stage of Ⅲ to Ⅳ, history of hypertension and diabetes mellitus, and elevated preoperative serum miR-128-3p expression were all independent risk factors for postoperative restenosis (P < 0.05).Pearson correlation analysis showed that preoperative serum miR-128-3p expression was significantly positively correlated with serum IL-6 or IL-8 levels (rs=0.646, 0.621, P < 0.001), negatively correlated with TIMP-1 levels (rs=-0.759, P < 0.001).
Conclusion Serum miR-128-3p level may be a specific biomarker of ISR in patients with LEAOD after endovascular intervention.
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