Objective To explore the application value of gene chip combined with T cell spot detection (T-SPOT) in diagnosis of pulmonary tuberculosis, and to analyze the detection efficiency of gene chip for drug resistance genes of Mycobacterium tuberculosis (MTB).
Methods A total of 108 patients with highly suspected pulmonary tuberculosis treated in the Department of Infection were selected as research subjects, all patients were diagnosed by genechip combined with T-SPOT at admission.Sputum tuberculosis culture experiment was used as the"gold standard "to evaluate the diagnostic efficacy of gene chip combined with T-SPOT technology in pulmonary tuberculosis.All confirmed patients were treated with anti-tuberculosis regimen, DNA sequencing was used as the" gold standard "to evaluate the detection efficiency of gene chip for MTB resistance genes.
Results Of 108 patients with highly suspected pulmonary tuberculosis, 100 cases were confirmed as pulmonary tuberculosis by sputum culture, accounting for 92.59%;the results of sputum tuberculosis culture were taken as the" gold standard ", the sensitivity and accuracy of T-SPOT combined with gene chip in the diagnosis of pulmonary tuberculosis were higher than that of single diagnosis, the difference was statistically significant (P < 0.05).The results of DNA sequencing were taken as the" gold standard", the sensitivity, specificity and accuracy of gene chip combined with T-SPOT for detecting MTB resistance genes were 92.31%, 97.37% and 96.08%.
Conclusion The sensitivity and accuracy of gene chip combined with T-SPOT in the diagnosis of pulmonary tuberculosis are high, gene chip has high detection efficiency in detecting MTB resistance genes.
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