Expression changes of interleukin-1α and interleukin-8 <i>in vitro</i> skin stimulation test of medical devices for medical devices with reconstructed human epidermis model

LIU Jia; FAN Chunguang; SUN Likui; ZHAO Zenglin

doi:10.7619/jcmp.20214815

Journal of Clinical Medicine in Practice > 2022 > 26(11): 18-21,33. > DOI: 10.7619/jcmp.20214815

LIU Jia, FAN Chunguang, SUN Likui, ZHAO Zenglin. Expression changes of interleukin-1α and interleukin-8 in vitro skin stimulation test of medical devices for medical devices with reconstructed human epidermis model[J]. Journal of Clinical Medicine in Practice, 2022, 26(11): 18-21,33. DOI: 10.7619/jcmp.20214815

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Expression changes of interleukin-1α and interleukin-8 in vitro skin stimulation test of medical devices for medical devices with reconstructed human epidermis model

Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection, National Medical Products Administration Key Laboratory for Safety Evaluation of Biomaterials and Medical Devices, Shandong Key Laboratory of Biological Evaluation for Medical Devices, Jinan, Shandong, 250101

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Received Date: December 06, 2021
Available Online: May 04, 2022

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Abstract

Abstract

Objective
To explore the skin irritation test in vitro as a substitute test for rabbit skin irritation to detect skin irritation, and to analyze the molecular markers related to the test model.
Methods
Two kinds of medical devices were selected, and skin irritation was detected by reconstructing human epidermis (RhE) model in vitro skin stimulation test. Skin tissue substrates were collected, and the contents of interleukin (IL) -1α and IL-8 in the substrate were determined by enzyme-linked immunosorbent assay (ELISA).
Results
The histopathology showed that the epithelial cells with 4% lactate were atrophied and the cuticle was exfoliated; there was no obvious abnormality in epithelial tissue by other methods. Epithelial cells were degenerate and necrotic in 1% sodium dodecyl sulfate (SDS) polar positive control with sodium chloride injection and 1% non-polar nonpolar positive control with sesame oil. ELISA results showed that IL-1α expression in substrate was positively correlated with skin irritation index, but IL-8 was negatively correlated with skin irritation index.
Conclusion
IL-1α can be used as a molecular marker for RhE model skin irritation test in vitro to analyze skin irritation of medical devices.
- reconstruction of human epidermis model,
- interleukin-1α,
- interleukin-8,
- skin irritation,
- medical device,
- molecular marker

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References

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Expression changes of interleukin-1α and interleukin-8 in vitro skin stimulation test of medical devices for medical devices with reconstructed human epidermis model

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