Objective To detect the methylation status of death-related protein kinase 1 (DAPK1), KLF4 and O6-methylguanine-DNA methyltransferase (MGMT) promoter region in cervical cancer tissues, and to preliminarily explore the relationship between their methylation status and human papillomavirus type 16 (HPV16) infection of cervical cancer.
Methods A total of 103 patients with cervical cancer were selected as cervical cancer group, and another 110 patients of chronic cervicitis with matched age in the same period were selected as control group. The methylation status of DAPK1, KLF4 and MGMT genes in cervical exfoliated tissues of the two groups were detected. HPV16 infection was detected by human papillomavirus, and the correlations of DAPK1, KLF4 and MGMT gene methylation with HPV16 infection were analyzed.
Results Compared with the control group, the positive rate of HPV16 infection in cervical cancer group was significantly increased (P < 0.05); compared with the control group, the abnormal methylation rates of DAPK1, KLF4 and MGMT genes in cervical cancer group were significantly increased (P < 0.05). The methylation rates of DAPK1, KLF4 and MGMT in cervical cancer tissues in patients with low differentiation, TNM stage Ⅲ to Ⅳ, lymph node metastases and distant metastases were significantly higher than those in patients with moderate to high differentiation, TNM stage Ⅰ to Ⅱ, without lymph node metastasis and without distant metastasis (P < 0.05). Compared with the negative HPV16 infection, the methylation rates of DAPK1, KLF4 and MGMT in HPV16 positive patients were increased significantly (P < 0.05). HPV16 infection was associated with methylation of DAPK1, KLF4 and MGMT (r=0.454, 0.497 and 0.307, P < 0.05).
Conclusion HPV16 infection may be related to the methylation of DAPK1, KLF4 and MGMT. HPV16 infection may promote the development of cervical cancer by influencing methylation of DAPK1, KLF4 and MGMT genes.
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