Drug resistance and efflux pump gene in Acinetobacter baumannii in department of geriatrics

  Objective  To observe the drug resistance of Acinetobacter baumannii to commonly used antibiotics in department of geriatrics and status of efflux pump gene-carrying so as to provide theoretical basis for prevention and control of nosocomial infection and clinical rational application of drugs.

  Methods  A total of 85 non-repetitive strains of Acinetobacter baumannii were collected and isolated from geriatric patients, disk dilution method was used to determine their drug sensitivity to 15 antibiotics commonly used in clinic. The adeB, adeG and adeJ genes-carrying were tested by polymerase chain reaction(PCR) method.

  Results  The sensitive rate of Acinetobacter baumannii was 100.0% to polymyxin B, 29.4% to minocycline, 35.3% to cefoperazone-sulbactam, 38.8% to amikacin and 41.2% to gentamicin, which had lower sensitive rates. Drug resistance rate of Acinetobacter baumannii to other detected antibiotics was over 50.0%, with 49 strains (57.6%) of multidrug-resistant bacteria. PCR results showed that the positive rates of adeB, adeG and adeJ genes in multi-drug resistant efflux pump gene-carrying Acinetobacter baumannii were 73.5%, 77.6% and 71.4%, respectively, which were significantly higher than those in non-multidrug-resistant bacteria (P < 0.05).

  Conclusion  The drug resistance of Acinetobacter baumannii in geriatrics is serious, and multi-drug resistance is related to carrying adeB, adeG and adeJ genes.