Objective To investigate the expression and clinical significance of microRNA-210(miR-210) and lipoprotein-associated phospholipase A2 (Lp-PLA2) in peripheral blood of patients with coronary heart disease.
Methods A total of 113 patients with coronary heart disease were selected as research objects, and were positive coronary angiography at admission(stenosis of vessel diameter of 50% and above). According to the classification of coronary heart disease, the patients were divided into acute myocardial infarction (AMI) group (42 cases), stable angina pectoris (SAP) group (37 cases) and unstable angina pectoris (UAP) group (34 cases). Another 106 patients with suspected coronary heart disease with negative coronary angiography (vessel diameter stenosis < 50%) during the same period were included in control group. Quantitative Real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the levels of miR-210, Lp-PLA2 and related indicators of cardiac functionStroke output (SV), cardiac index (CI) and left ventricular ejection fraction (LVEF), degree of coronary artery disease (Gensini score) in peripheral blood within 24 h after admission without drug treatment, and were conducted Pearson correlation analysis. Multivariate Logistic regression analysis was used to explore the main risk factors of coronary heart disease.
Results The relative expressions of miR-210 and Lp-PLA2 in peripheral blood of the AMI group, SAP group and UAP group were significantly higher than those of the control group, while the cardiac function indexes were significantly lower than those of the control group(P < 0.05). The results of Pearson correlation analysis showed that miR-210 in peripheral blood of patients with coronary heart disease was positively correlated with Lp-PLA2 (P < 0.05), and both of them were negatively correlated with SV, CI and LVEF, and positively correlated with Gensini score of coronary artery disease degree (P < 0.05). Multivariate stepwise Logistic regression analysis showed that miR-210 and Lp-PLA2 were independent risk factors for patients with coronary heart disease.
Conclusion MiR-210 and Lp-PLA2 in peripheral blood are closely related to the occurrence and development of coronary heart disease. They can predict the potential risk of coronary heart disease, and have positive significance for the early diagnosis, treatment and prognosis evaluation of coronary heart disease.
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