LI Haixia, TAO Yongming, ZHAO Qiuxia, WANG Lihe, GAO Xiaopeng. Study on the correlation between regulatory T cells and EB virus DNA concentration in children with infectious mononucleosis[J]. Journal of Clinical Medicine in Practice, 2021, 25(15): 98-101, 105. DOI: 10.7619/jcmp.20211592
Citation: LI Haixia, TAO Yongming, ZHAO Qiuxia, WANG Lihe, GAO Xiaopeng. Study on the correlation between regulatory T cells and EB virus DNA concentration in children with infectious mononucleosis[J]. Journal of Clinical Medicine in Practice, 2021, 25(15): 98-101, 105. DOI: 10.7619/jcmp.20211592

Study on the correlation between regulatory T cells and EB virus DNA concentration in children with infectious mononucleosis

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  • Received Date: April 13, 2021
  • Available Online: September 27, 2021
  • Published Date: August 14, 2021
  •   Objective  To investigate the changes of regulatory T cell levels in children with infectious mononucleosis (IM) and its correlation with the concentration of Epstein-Barr virus DNA (EBV-DNA).
      Methods  Forty-two children with IM were selected as observation group, and thirty healthy children as control group. The fasting venous blood samples of two groups were collected, and the expression values of CD3+, CD4+, CD8+, CD4+CD25+, CD4+CD25+CD127- were determined by flow cytometry, and the EBV-DNA concentration was determined by Polymerase Chain Reaction (PCR). The test results of the two groups were compared, and the correlation between the level of regulatory T cells and the concentration of EBV-DNA was analyzed.
      Results  The CD3+ and CD8+ levels of the observation group were significantly higher than those of the control group, and the CD4+, CD4+/CD8+, CD4+CD25+, CD4+CD25+CD127- levels were significantly lower than those of the control group (P < 0.05). The positive detection rate of EBV-DNA in the observation group was 85.71%, which was significantly higher than 3.33% in the control group (P < 0.05). The EBV-DNA content in the acute phase of the observation group was (76.62±5.10)×103 copies/mL, and the EBV-DNA content in the recovery phase was (0.68±2.30)×103 copies/mL. EBV-DNA concentration in the acute phase of IM children was positively correlated with CD3+ and CD8+ levels, and negatively correlated with CD4+, CD4+/CD8+, CD4+CD25+, CD4+CD25+CD127- levels.
      Conclusion  The level of regulatory T cells in children with IM is negatively correlated with the concentration of EBV-DNA, suggesting that there is a general immune regulatory dysfunction in children with IM in the acute phase, and the degree of immune dysfunction is closely related to the amount of EB virus infection.
  • [1]
    陈树霞. 小儿传染性单核细胞增多症68例临床特点分析[J]. 世界复合医学, 2019, 5(12): 104-106. https://www.cnki.com.cn/Article/CJFDTOTAL-SJFH201912034.htm
    [2]
    胡振华, 樊一笋, 汪浩, 等. 外周血CD4-CD8-双阴性T细胞在小儿传染性单核细胞增多症患者中的表达[J]. 国际免疫学杂志, 2019, 42(5): 486-489. doi: 10.3760/cma.j.issn.1673-4394.2019.05.008
    [3]
    赵青松, 方瑞, 刘亢亢, 等. CD4+ CD25+调节性T淋巴细胞在儿童传染性单核细胞增多症的变化及作用[J]. 安徽医学, 2019, 40(2): 175-177. doi: 10.3969/j.issn.1000-0399.2019.02.018
    [4]
    叶冬梅, 张志伟, 刘勇. 传染性单核细胞增多症的病理分期与鉴别诊断[J]. 中华病理学杂志, 2019, 48(05): 421-424.
    [5]
    樊亚楠, 施逸怡, 徐京杭, 等. 274例青少年和成人传染性单核细胞增多症患者肝损伤临床特征分析[J]. 临床肝胆病杂志, 2021, 37(3): 636-641. doi: 10.3969/j.issn.1001-5256.2021.03.026
    [6]
    高荣理, 陈垂婉, 蔡翠珠, 等. EBV合并CMV感染的传染性单核细胞增多症患者T细胞免疫状态及炎症指标变化[J]. 中华医院感染学杂志, 2020, 30(19): 2975-2978. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHYY202019022.htm
    [7]
    SAKHARNOV N A, UTKIN O V, FILATOVA E N, et al. Apoptosis-and survival-related gene mRNA profile in peripheral blood leukocytes in children with acute EBV infectious mononucleosis[J]. Iimm, 2020, 9(5/6): 723-734.
    [8]
    HOSAKA S, KOBAYASHI C, SAITO H, et al. Establishment of immunity against Epstein-Barr virus infection in a patient with CHARGE/complete DiGeorge syndrome after peripheral blood lymphocyte transfusion[J]. Pediatr Transplant, 2019, 23(4): e13424. doi: 10.1111/petr.13424
    [9]
    张莉, 朱红胜, 马钧, 等. 血清ADA、LDH及外周血淋巴细胞亚群比例联合检测对传染性单核细胞增多症的诊断价值[J]. 检验医学, 2020, 35(9): 881-884. doi: 10.3969/j.issn.1673-8640.2020.09.006
    [10]
    祁正红, 陈雪夏, 林先耀, 等. 传染性单核细胞增多症患儿外周血淋巴细胞亚群、细胞因子、TLRs的表达变化及其临床意义[J]. 中国现代医学杂志, 2020, 30(12): 51-56. doi: 10.3969/j.issn.1005-8982.2020.12.009

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