Objective To investigate the basophil activation situation of patients being allergic to pollen of phoenix roebelenii and fishtail palm.
Methods A total of 83 patients being allergic to pollen of phoenix roebelenii and fishtail palm were chosen as observation group, 83 healthy persons at the same time were chosen as control group. The fluorescence intensity of basophil activation situation(CD63, CD203c and CD45)of two groups were compared, and the detection results of observation group with different allergens, kinds and UniCAP classifications were compared, and the relationships between the fluorescence intensity of basophil activation indexes and UniCAP classifications were analyzed by Spearman rank correlation analysis.
Results The fluorescence intensities of basophil activation of CD63, CD203c and CD45 of the observation group were(32.26±2.23), (50.31±5.61) and (36.36±5.03), respectively, and they were significantly higher than (6.15±1.20), (18.28±2.63) and (7.02±1.36) of the control group(P < 0.05). There were no statistically significant differences in detection results of the observation group with different allergens and kinds (P>0.05). The fluorescence intensities of basophil activation CD63, CD203c and CD45 of the observation group with UniCAP classifications 5 to 6 were(38.16±3.15), (66.73±6.96) and (50.86±6.59), respectively, which were significantly higher than (29.37±2.31), (51.13±5.78) and(39.10±5.13) of UniCAP classifications 3 to 4 and (18.68±1.76), (29.36±3.79) and (22.32± 1.93) of 1 to 2 grading of UniCAP classification. The Spearman rank correlation analysis showed the basophil activation indexes such as CD63, CD203c and CD45 were positively correlated with UniCAP classifications(P < 0.05).
Conclusion The basophil activation situation of patients being allergic to pollen of phoenix roebelenii and fishtail palm are obvious, and the expression degree are close to the UniCAP classifications, so the detection value are higher.
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