Role of Actinidia Chinensis Planch root extract in radiosensitization for hepatoma cell Bel-7402

Objective To explore the role of Actinidia Chinensis Planch root extract in radiosensitization for hepatoma cell Bel-7402. Methods Flow cytometry was used to analyze the effect of Actinidia Chinensis Planch root extract on apoptosis and morphology of hepatoma cell line Bel-7402. The cell survival fraction of Bel-7402 was measured by colony-forming method, and the cell survival curve was drawn. Results Flow cytometry showed that the proportion of apoptotic cells of Bel-7402 in the combined radiotherapy group was significantly higher than that in the radiotherapy group and the drug group. According to the analysis of the distribution of apoptotic cells, the drug mainly caused the apoptosis and necrosis of Bel-7402 cells in the middle and late stage, but had no significant effect on apoptosis of cells in early stage. Compared with the radiotherapy group, the number of normal cells in the combined radiotherapy group decreased gradually, while the number of apoptotic cells increased gradually. Compared with the radiotherapy group, the cell survival curve of the combined radiotherapy group showed that the cell survival fraction was significantly reduced, which indicated that the extract of Actinidia Chinensis Planch root had the radiosensitization effect on Bel-7402 cells. Conclusion Actinidia Chinensis Planch root extract may produce radiosensitization effect on Bel-7402 cells by inducing apoptosis of cells, and its mechanism may be involved in blocking tumor cells in G₁/M phase and cause DNA damage.