The Construction and authentication PIAS-1 gene expression of eukaryotic plasmid

Objective To construct interference vector of Plko.1 and PIAS-1.Methods Sequence of siRNA targeted by PIAS-1 was designed and was cloned into the expression vector pLKO. 1.The recombinants were identified by gene sequencing.Results PCR test and gene sequencing a-nalysis showed that the recombinant plasmid was constructed correctly.Conclusion Sequence of siR-NA targeted by PIAS-1 has been constructed successfully,and it provides stable transfection of RNA interference vector for the proliferation and apoptosis of PIAS-1 in cervical cancer.