抗HCMV不同多肽的6株McAb的研究

严华; 李国才; 朱网娣

抗HCMV不同多肽的6株McAb的研究

STUDY ON MONOCLONAL ANTIBODY AGAINST DIFFERENTHUMAN CYTOMEGALOVIRUS POLYPEPTIDES

摘要

摘要: 目有：为了制备人巨细胞病毒（HCMV）单特异性可溶性多肽的单克隆抗体。方法：采用细胞杂交技术建立了分泌单克隆抗体（McAb）的13株杂交瘤细胞，本文选取6株杂交癌细胞作了进一步研究。结果：6株杂交瘤细胞分泌的McAb均为IgGl，重链、轻链分子量分别为50KD、25KD。间接免疫荧光试验表明：McAb8B8相应的多肽为即刻早期抗原；而McAb7B4、7D7、7E7、7E11、8D6的相应病毒多肽为晚期抗原。免疫印迹试验表明：McAb7B4、7D7、7E7、7E11、8B8、8D6相应的病毒多肽分子量分别为46、150、38、51、72、65KDO结论：6株McAb可用作HCMV感染的诊断。

Abstract: Aim:Nonionic detergent solubilised Polypepdides from human cytomegalovirus virions. Were used as im-munogens to preduce monoclonal antibodies (McAb). Method: 13 hybrid cells which secrete hybrdization technique McAbs a-gainst different human cytomegalovirus (HCMV) polypeptides were established by cell fusion. 6 hybrid cells (7B4. 7D7.7E7. 7E11.8B8. 8D6) were selected for further. Results: All subclass of the 6 McAb are IgG1 type (molecular weight of heavy chain and light chain is separately 50KD. 25KD). Indrect immunofluorescence reation of 6 McAb with HCMV infected cells at different time showed that McAb 8B8 is against the immediate early antigen of HCMV, while McAb 7B4' 7D7. 7E7.7E11. 8D6 are against the late antigen of HCMV. Immunoblot analysis revealed 6 McAb are directed against different human cytomegalovirus polypeptides with molecular weight of approximately 46. 150. 38. 51.72.65 KD. Conclusion: 6 McAb may by used to diagnose HCMV.

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