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多溴蛋白1在前列腺癌组织中的表达及其与CD8+ T淋巴细胞浸润的关系

Expression of polybromo 1 in prostate cancer tissues and its relationship with CD8+ T lymphocyte infiltration

    摘要
  • 摘要:
    目的 探讨前列腺癌组织中多溴蛋白1(PBRM1)的表达情况及其与CD8+ T淋巴细胞浸润的相关性。
    方法 选取124例前列腺癌患者纳入观察组，另选取同期80例前列腺良性肿瘤患者纳入对照组。分别采用免疫组织化学染色法(免疫组化法)、实时荧光定量聚合酶链反应(qRT-PCR)及蛋白质印迹法(Western blot)检测2组患者肿瘤组织及观察组肿瘤邻近正常组织中PBRM1表达情况，并采用流式细胞术检测组织中CD8+和CD4+ T淋巴细胞浸润情况。
    结果 观察组肿瘤组织中PBRM1阳性表达率为59.68%, 低于对照组的85.00%和观察组肿瘤邻近正常组织的81.45%, 差异有统计学意义(P < 0.001); 观察组肿瘤组织中PBRM1 mRNA和PBRM1蛋白相对表达量以及CD8+ T淋巴细胞浸润百分比均低于对照组肿瘤组织、观察组肿瘤邻近正常组织，差异有统计学意义(P < 0.05)。Pearson相关分析结果显示，肿瘤组织中PBRM1蛋白相对表达量与CD8+ T淋巴细胞浸润百分比呈正相关(r＝0.856, P < 0.001)。观察组中，肿瘤最大径≥3 cm、低分化、临床分期Ⅲ~Ⅳ期、Gleason评分≥8分、前列腺特异性抗原(PSA)≥20 ng/mL患者的PBRM1蛋白相对表达量分别低于最大径 < 3 cm、中高分化、临床分期Ⅰ~Ⅱ期、Gleason评分 < 8分、PSA < 20 ng/mL患者，差异有统计学意义(P < 0.05)。
    结论 前列腺癌组织中PBRM1表达下调可能与肿瘤恶性程度增加及CD8+ T淋巴细胞浸润减少相关，进而增强了肿瘤的免疫逃逸能力。

     

    Abstract:
    Objective To investigate the expression of polybromo 1 (PBRM1) in prostate cancer tissues and its correlation with CD8+ T lymphocyte infiltration.
    Methods A total of 124 prostatecancer patients were enrolled as observation group, and 80 patients with benign prostate tumors during the same period were selected as control group. Immunohistochemical staining (IHC), real-time fluorescent quantitative polymerase chain reaction (qRT-PCR), and western blot were used to detect the expression of PBRM1 in the tumor tissues of both groups and the adjacent normal tissues of the observation group (more than 1 cm from the tumor edge). Flow cytometry was employed to detect the infiltration of CD8+ and CD4+ T lymphocytes in the tissues.
    Results The positive expression rate of PBRM1 in the tumor tissues of the observation group was 59.68%, which was significantly lower than 85.00% in the control group and 81.45% in the adjacent normal tissues of the observation group (P < 0.001). The relative expression levels of PBRM1 mRNA and PBRM1 protein, as well as the percentage of CD8+ T lymphocyte infiltration in the tumor tissues of the observation group were all lower than those in the tumor tissues of the control group and the adjacent normal tissues of the observation group (P < 0.05). Pearson correlation analysis revealed a positive correlation between the relative expression level of PBRM1 protein and the percentage of CD8+ T lymphocyte infiltration in the tumor tissues (r＝0.856, P < 0.001). In the observation group, patients with tumors having a maximum diameter ≥3 cm, poor differentiation, clinical stage Ⅲ to Ⅳ, Gleason score ≥8, and prostate-specific antigen (PSA)≥20 ng/mL had significantly lower relative expression levels of PBRM1 protein compared to those with tumors having a maximum diameter < 3 cm, moderate to well differentiation, clinical stage Ⅰ to Ⅱ, Gleason score < 8, and PSA < 20 ng/mL, and the differences were statistically significant (P < 0.05).
    Conclusion Downregulation of PBRM1 expression in prostate cancer tissues may be associated with an increase in tumor malignancy and a decrease in CD8+ T lymphocyte infiltration, thereby enhancing the tumor′s immune escape ability.

     

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