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人类胎盘基因CpG岛甲基化水平与自然流产的相关性

Correlation between CpG island methylation levels of human placental genes and spontaneous abortion

    摘要
  • 摘要:
    目的 探讨人类胎盘基因CpG岛甲基化水平与孕妇自然流产的相关性。
    方法 选取接受常规产前检查的孕妇55例为研究对象，其中25例自然流产(流产组)，30例正常分娩(对照组)。收集入组孕妇的临床资料及人类胎盘组织样本。比较2组临床一般资料。分析相关胎盘基因的甲基化水平对孕妇自然流产结局的预测价值。采用Logistic回归分析法分析孕妇自然流产的影响因素。
    结果 2组在孕妇年龄、流产儿/新生儿出生体质量、孕妇妊娠天数和流产儿/新生儿性别方面比较，差异有统计学意义(P＜0.05)。MECP2-1、MECP2-4、HSD11B2、MECP2-3 和 MECP2-2 基因甲基化与自然流产相关。流产组的 MECP2-1、MECP2-4、HSD11B2 和 MECP2-3 基因甲基化率高于对照组，差异有统计学意义(P＜0.05)。 MECP2-1、MECP2-4、HSD11B2、MECP2-3 和 MECP2-2 的曲线下面积(AUC)分别为0.773、0.737、0.700、0.663和0.627。Logistic回归分析结果显示, MECP2-1 是孕妇发生流产的独立影响因素(P＜0.05)。
    结论 MECP2-1、MECP2-4、HSD11B2、MECP2-3 和 MECP2-2 基因甲基化水平和孕妇的自然流产结局有关。MECP2-1 基因是自然流产发生的独立影响因素。

     

    Abstract:
    Objective To investigate the correlation between CpG island methylation levels of human placental genes and spontaneous abortion in pregnant women.
    Methods A total of 55 pregnant women who underwent routine prenatal examination were selected as the study subjects, including 25 cases of spontaneous abortion (abortion group) and 30 cases of normal delivery (control group). Clinical data and human placental tissue samples were collected from the enrolled pregnant women. Clinical general data of the two groups were compared. The predictive value of methylation levels of related placental genes on the outcome of spontaneous abortion in pregnant women was analyzed. Logistic regression analysis was used to analyze the influencing factors of spontaneous abortion in pregnant women.
    Results There were statistically significant differences in maternal age, birth weight of aborted fetuses/neonates, days of pregnancy, and gender of aborted fetuses/neonates between the two groups (P < 0.05). Methylation of MECP2-1, MECP2-4, HSD11B2, MECP2-3 and MECP2-2 genes was associated with spontaneous abortion. The methylation rates of MECP2-1, MECP2-4, HSD11B2 and MECP2-3 genes in the abortion group were higher than those in the control group, with statistically significant differences (P < 0.05). The area under the curve (AUC) of MECP2-1, MECP2-4, HSD11B2, MECP2-3 and MECP2-2 was 0.773, 0.737, 0.700, 0.663, and 0.627, respectively. Logistic regression analysis showed that MECP2-1 was an independent influencing factor for miscarriage in pregnant women (P < 0.05).
    Conclusion The methylation levels of MECP2-1, MECP2-4, HSD11B2, MECP2-3 and MECP2-2 genes are related to the outcome of spontaneous abortion in pregnant women. The MECP2-1 gene is an independent influencing factor for spontaneous abortion.

     

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