Diagnostic efficacy of serum microRNA-375 and microRNA-760 combined with liver enhancement computed tomography in hepatocellular carcinoma
-
摘要:目的
探讨血清微小RNA-375(miR-375)、微小RNA-760(miR-760)联合肝脏增强CT技术对肝细胞癌(HCC)的诊断效能。
方法选取144例HCC患者纳入癌症组，另选取同期144例非肿瘤肝病患者纳入良性组，检测2组患者血清miR-375、miR-760表达水平。绘制受试者工作特征(ROC)曲线，分析血清miR-375、miR-760表达水平联合肝脏增强CT对HCC的诊断价值。采用Kappa检验分析血清miR-375、miR-760表达水平和肝脏增强CT单独及联合诊断HCC与“金标准”病理结果的一致性。
结果癌症组患者血清miR-375、miR-760表达水平均低于良性组，差异有统计学意义(P<0.05)。HCC患者血清miR-375、miR-760表达水平均与肿瘤数目、肿瘤直径、TNM分期、远处器官转移、分化程度、门静脉癌栓、乙型肝炎表面抗原相关(P<0.05)。ROC曲线分析结果显示，血清miR-375、miR-760和肝脏增强CT联合诊断HCC的曲线下面积(AUC)显著大于三者单独诊断的AUC(P<0.05), 与病理结果的一致性极高(Kappa=0.826)。
结论HCC患者血清miR-375、miR-760表达水平低于非肿瘤肝病患者，血清miR-375、miR-760水平和肝脏增强CT技术三者联用可显著提升对HCC的诊断效能。
Abstract:ObjectiveTo investigate the diagnostic efficacy of serum microRNA-375 (miR-375) and microRNA-760 (miR-760) combined liver enhancement computed tomography(CT) in hepatocellular carcinoma (HCC).
MethodsA total of 144 patients with HCC were included in cancer group, and another 144 patients with benign liver tumors in the same period were included in benign group. The expression levels of serum miR-375 and miR-760 were measured in both groups. Receiver operating characteristic (ROC) curves were plotted to analyze the diagnostic value of serum miR-375 and miR-760 expression levels combined with liver enhancement CT in HCC. The Kappa test was used to analyze the consistency of lonely detection of serum miR-375, miR-760 levels and liver enhancement CT, and their combined diagnosis for HCC with the gold standard pathological results.
ResultsThe expression levels of serum miR-375 and miR-760 in the cancer group were significantly lower than those in the benign group (P<0.05). The expression levels of serum miR-375 and miR-760 in patients with HCC were correlated with the number of tumor, tumor diameter, TNM stage, distant organ metastasis, degree of differentiation, portal vein tumor thrombus, and hepatitis B surface antigen (P<0.05). The ROC curve analysis showed that the area under the curve (AUC) of combined diagnosis of HCC using serum miR-375, miR-760, and liver enhancement CT was significantly greater than the AUC of individual diagnosis of the three methods (P<0.05), and there was a high consistency with pathological results (Kappa=0.826).
ConclusionThe expression levels of serum miR-375 and miR-760 in patients with HCC are lower than those in patients with benign liver tumors. The combined use of serum miR-375 and miR-760 levels and liver enhancement CT technology can significantly improve the diagnostic efficacy of HCC.
-
-
表 1 引物序列
基因 上游引物 下游引物 miR-375 5′-AGCCGTTTGTTCGTTCGGCT-3′ 5′-GTGCAGGGTCCGAGGT-3′ miR-760 5′-AATCTGGTGGCTGTGCACAC-3′ 5′-CCTTGGGCTTGAATGGAGT-3′ U6 5′-CATCCGATAA AATTGGAACGA-3′ 5′-TTTGTGCGTGTCATCCTTGCG-3′ miR-375: 微小RNA-375; miR-760: 微小RNA-760。 
下载: 导出CSV
表 2 肝脏增强CT诊断HCC与病理诊断结果的一致性分析
肝脏增强CT 病理诊断结果 Kappa P 肝细胞癌 良性肝肿瘤 肝细胞癌 101 11 0.625 ＜0.001 良性肝肿瘤 43 133 合计 144 144
下载: 导出CSV
表 3 miR-375、miR-760表达水平与HCC患者病理特征的关系($\overline x $ ±s)
病理特征 分类 n miR-375 miR-760 表达水平 t P 表达水平 t P 性别 男 83 0.85±0.19 1.631 0.105 0.80±0.17 0.786 0.433 女 61 0.80±0.17 0.78±0.12 年龄 ＜50岁 76 0.85±0.20 1.315 0.191 0.81±0.16 1.542 0.125 ≥50岁 68 0.81±0.16 0.77±0.15 肿瘤数目 单发 82 0.86±0.19 2.289 0.024 0.82±0.14 2.793 0.006 多发 62 0.79±0.17 0.75±0.16 肿瘤直径 ＜5 cm 95 0.87±0.17 3.776 ＜0.001 0.83±0.17 4.404 ＜0.001 ≥5 cm 49 0.75±0.20 0.71±0.12 TNM分期 Ⅰ~Ⅱ期 89 0.89±0.20 5.109 ＜0.001 0.85±0.16 6.248 ＜0.001 Ⅲ期 55 0.73±0.15 0.69±0.13 远处器官转移 有 118 0.81±0.16 2.834 0.005 0.77±0.14 3.077 0.003 无 26 0.92±0.25 0.87±0.19 分化程度 中/高分化 98 0.85±0.18 2.214 0.028 0.81±0.16 2.591 0.011 低分化 46 0.78±0.17 0.74±0.13 门静脉癌栓 有 31 0.74±0.13 3.031 0.003 0.71±0.12 3.236 0.002 无 113 0.85±0.19 0.81±0.16 乙型肝炎表面抗原 阳性 111 0.81±0.19 2.522 0.013 0.77±0.13 2.991 0.003 阴性 33 0.90±0.14 0.86±0.21
下载: 导出CSV
-
[1] VOGEL A, MEYER T, SAPISOCHIN G, et al. Hepatocellular carcinoma[J]. Lancet, 2022, 400(10360): 1345-1362. doi: 10.1016/S0140-6736(22)01200-4
[2] 王丽菲, 罗龙龙, 郑英, 等. 巨噬细胞移动抑制因子在肝细胞癌中的研究进展[J]. 实用临床医药杂志, 2023, 27(14): 126-130. doi: 10.7619/jcmp.20231220 [3] 彭敏. 动态增强CT诊断肝细胞癌的影像表现及应用价值[J]. 现代诊断与治疗, 2021, 32(20): 3311-3312. [4] 刘向东, 闫松果, 孙世松. 动态增强CT扫描与MRI对肝细胞癌的诊断价值及影像学特征分析[J]. 实用癌症杂志, 2023, 38(1): 89-91. doi: 10.3969/j.issn.1001-5930.2023.01.025 [5] WEI J H, LU Y R, WANG R Q, et al. MicroRNA-375: potential cancer suppressor and therapeutic drug[J]. Biosci Rep, 2021, 41(9): BSR20211494. doi: 10.1042/BSR20211494
[6] JOKOVIC S M, DOBRIJEVIC Z, KOTARAC N, et al. MiR-375 and miR-21 as potential biomarkers of prostate cancer: comparison of matching samples of plasma and exosomes[J]. Genes, 2022, 13(12): 2320. doi: 10.3390/genes13122320
[7] MANVATI M K S, KHAN J, VERMA N, et al. Association of miR-760 with cancer: an overview[J]. Gene, 2020, 747: 144648. doi: 10.1016/j.gene.2020.144648
[8] HUANG X J, LAI S Q, QU F L, et al. CCL18 promotes breast cancer progression by exosomal miR-760 activation of ARF6/Src/PI3K/Akt pathway[J]. Mol Ther Oncolytics, 2022, 25: 1-15. doi: 10.1016/j.omto.2022.03.004
[9] 黄伟康, 文戈, 吴水天, 等. CT扫描联合血清AFP-L3、CEA在诊断肝细胞癌中的应用价值分析[J]. 医学影像学杂志, 2021, 31(5): 810-813. [10] 王莉琳, 张永宏, 陈新月. 2010年美国肝病年会(AASLD)肝细胞癌诊疗指南[J]. 北京医学, 2011, 33(3): 236-251. [11] 郑可国, 许达生, 李子平. 肝细胞癌临床CT诊断[M]. 广州: 广东世界图书出版公司, 2003: 22-67. [12] CALDERARO J, SERAPHIN T P, LUEDDE T, et al. Artificial intelligence for the prevention and clinical management of hepatocellular carcinoma[J]. J Hepatol, 2022, 76(6): 1348-1361. doi: 10.1016/j.jhep.2022.01.014
[13] SUGAWARA Y, HIBI T. Surgical treatment of hepatocellular carcinoma[J]. Biosci Trends, 2021, 15(3): 138-141. doi: 10.5582/bst.2021.01094
[14] LLOVET J M, BAERE T D, KULIK L, et al. Locoregional therapies in the era of molecular and immune treatments for hepatocellular carcinoma[J]. Nat Rev Gastroenterol Hepatol, 2021, 18(5): 293-313. doi: 10.1038/s41575-020-00395-0
[15] 吴建峰, 李林静, 方春, 等. 个体化碘对比剂注射速率方案在肝脏增强CT中提高增强质量一致性的价值[J]. 中国医师杂志, 2022, 24(3): 456-458. doi: 10.3760/cma.j.cn431274-20210518-00556 [16] LIU C F, LI J, WANG W, et al. MiR-206 inhibits liver cancer stem cell expansion by regulating EGFR expression[J]. Cell Cycle, 2020, 19(10): 1077-1088. doi: 10.1080/15384101.2020.1739808
[17] LIU Y, WANG Q Y, WEN J, et al. MiR-375: a novel multifunctional regulator[J]. Life Sci, 2021, 275: 119323. doi: 10.1016/j.lfs.2021.119323
[18] NI H W, QIN H, SUN C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis[J]. Stem Cell Res Ther, 2021, 12(1): 325. doi: 10.1186/s13287-021-02394-7
[19] WANG C, LUO J, CHEN Z H, et al. MiR-375 impairs the invasive capabilities of hepatoma cells by targeting HIF1α under hypoxia[J]. Dig Dis Sci, 2021, 66(2): 493-502. doi: 10.1007/s10620-020-06202-9
[20] XU R, YIN S W, ZHENG M, et al. Circular RNA circZFR promotes hepatocellular carcinoma progression by regulating miR-375/HMGA2 axis[J]. Dig Dis Sci, 2021, 66(12): 4361-4373. doi: 10.1007/s10620-020-06805-2
[21] WU X D, CHEN H, LIU N, et al. Curcumin suppresses lung cancer progression via circRUNX1 mediated miR-760/RAB3D axis[J]. Thorac Cancer, 2023, 14(5): 506-516. doi: 10.1111/1759-7714.14773
[22] WANG Q H, WANG G, XU X D, et al. MiR-760 mediated the proliferation and metastasis of hepatocellular carcinoma cells by regulating HMGA2[J]. Pathol Res Pract, 2021, 222: 153420. doi: 10.1016/j.prp.2021.153420
[23] YIN L N, SUN T T, LIU R B. NACC-1 regulates hepatocellular carcinoma cell malignancy and is targeted by miR-760[J]. Acta Biochim Biophys Sin, 2020, 52(3): 302-309. doi: 10.1093/abbs/gmz167
[24] LI J F, QIN Y Y, WANG W J, et al. Peiminine inhibits the progression of colorectal cancer through up-regulating miR-760 via declining the expression of long noncoding RNA LINC00659[J]. Anticancer Drugs, 2021, 32(2): 148-156. doi: 10.1097/CAD.0000000000000981
计量
- 文章访问数: 80
- HTML全文浏览量: 9
- PDF下载量: 11
苏公网安备 32100302010246号