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异丙肾上腺素诱导小鼠急性心肌梗死模型制备方法研究

Preparation method of acute myocardial infarction model induced by isoproterenol in mice

    摘要
  • 摘要:
    目的 探讨异丙肾上腺素给药方式对小鼠心肌损伤的影响，建立小鼠急性心肌梗死（AMI）模型。
    方法 小鼠皮下注射异丙肾上腺素（150 mg/kg），于不同时点取小鼠血样，检测血清中AMI相关指标肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、心肌肌钙蛋白T（cTnT）、乳酸脱氢酶（LDH）、谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素6（IL-6）、降钙素原（PCT）和C反应蛋白（CRP）的含量变化；通过苏木素-伊红染色观察小鼠心脏组织的病理改变。
    结果 造模后，血清中CK、CK-MB、LDH、cTnT、ALT、AST水平升高（P < 0.05或P < 0.01）；2次连续给予异丙肾上腺素，小鼠血清中AMI相关指标升高，且相关指标在给药后16 h达到最高浓度（P < 0.05或P < 0.01）。苏木素-伊红染色结果显示，肌组织发生病理学改变。
    结论 皮下注射异丙肾上腺素可诱导小鼠AMI，其中连续2次注射后16 h心肌损伤最为严重，这为AMI的临床治疗药物研究和创新药物开发奠定基础。

     

    Abstract:
    Objective To investigate the effects of isoproterenol administration on myocardial injury and establish acute myocardial infarction (AMI) model in mice.
    Methods Mice were injected subcutaneously with isoproterenol (150 mg/kg), and blood samples were taken at different time points. The contents of AMI related indexes creatine kinase (CK), creatine kinase isoenzyme (CK-MB), cardiac troponin T (cTnT), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL-6), procalcitonin (PCT) and C-reactive protein (CRP) in serum were detected. The pathological changes of cardiac tissue were observed by hematoxylin-eosin staining.
    Results The serum levels of CK, CK-MB, LDH, cTnT, ALT and AST were significantly increased after isoproterenol model (P < 0.05, P < 0.01); after two consecutive administrations of isoproterenol, the serum AMI-related indexes increased significantly, and the relevant indexes reached the highest concentration 16 h after administration (P < 0.05, P < 0.01). Hematoxylin-eosin staining results showed that pathological changes occurred in myocardial tissue.
    Conclusion Subcutaneous injection of isoproterenol induces AMI injury. The AMI injury at 16 h after two consecutive injections is the most serious, which lays a foundation for clinical drug research and innovative drug development of AMI.

     

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