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系统性红斑狼疮患者外周血中γδT细胞及其主要功能亚群的变化及临床意义

Changes and clinical significance of γδT cells and their major functional subsets in peripheral blood of patients with systemic lupus erythematosus

    摘要
  • 摘要:
    目的 探讨系统性红斑狼疮(SLE)患者外周血中γδT细胞及其主要功能亚群γδT1、γδT17细胞的变化及其临床意义。
    方法 采用流式细胞术检测21例SLE患者(SLE组)、16例健康对照患者(HC组)外周血γδT细胞、γδT1细胞、γδT17细胞的比例及绝对数。采用Pearson相关性分析法分析外周血γδT细胞、γδT1细胞、γδT17细胞与临床指标的相关性。
    结果 SLE患者外周血γδT细胞占淋巴细胞百分率为(2.30±1.10)%, 低于HC组的(3.30±1.51)%, 差异有统计学意义(P < 0.05); γδT1细胞占淋巴细胞百分率为(0.93±0.67)%, 低于HC组的(2.09±1.21)%, 差异有统计学意义(P < 0.001); γδT17细胞占淋巴细胞比率为(1.53±2.82)‰, 与HC组的(0.18±0.12)‰比较有增多的趋势，但差异无统计学意义(P>0.05)。SLE患者外周血γδT细胞绝对数为(2.88±2.18)×104/mL, 低于HC组的(7.96±3.96)×104/mL, 差异有统计学意义(P < 0.000 1); SLE患者外周血γδT1细胞绝对数为(1.20±1.17)×104/mL, 低于HC组的(5.05±3.04)×104/mL, 差异有统计学意义(P < 0.000 1); SLE患者外周血γδT17细胞绝对数为(1.03±1.08)×103/mL, 高于HC组的(0.40±0.24)×103/mL, 差异有统计学意义(P < 0.05)。SLE患者外周血中γδT17细胞比例与系统性红斑狼疮疾病活动度评分(SLEDAI)(r=0.59, P < 0.01)、红细胞沉降率(r=0.65, P < 0.01)呈正相关, γδT17细胞绝对数与SLEDAI(r=0.59, P < 0.01)呈正相关, 与补体C3水平(r=-0.53, P < 0.05)呈负相关。
    结论 SLE患者外周血中γδT细胞数量显著下降主要由其γδT1细胞亚群减少所致, γδT17细胞在SLE发病及活动中发挥了重要作用。

     

    Abstract:
    Objective To investigate the changes and clinical significance of γδT cells and their major functional subsets γδT1 and γδT17 cells in the peripheral blood of patients with systemic lupus erythematosus (SLE).
    Methods Ratios and absolute numbers of γδT cells, γδT1 cells and γδT17 cells in peripheral blood were detected by flow cytometry in 21 SLE patients (SLE) group and 16 healthy controls (HC group). Correlations of γδT cells, γδT1 cells and γδT17 cells in the peripheral blood with clinical indicators were analyzed by Pearson correlation analysis.
    Results In SLE patients, the percentage of γδT cells to lymphocytes in the peripheral blood was (2.30±1.10)%, which was significantly lower than (3.30±1.51)% in the HC group (P < 0.05); the percentage of γδT1 cells to lymphocytes in peripheral blood was (0.93±0.67)%, which was significantly lower than (2.09±1.21)% in the HC group (P < 0.001); the ratio of γδT17 cells to lymphocytes in the peripheral blood was (1.53±2.82)‰, which showed an increasing trend when compared to (0.18±0.12)‰ in the HC group (P>0.05). The absolute number of γδT cells in the peripheral blood in SLE patients was (2.88±2.18)×104/mL, which was significantly lower than (7.96±3.96)×104/mL in the HC group (P < 0.000 1); the absolute number of γδT1 cells in the peripheral blood in SLE patients was (1.20±1.17)×104/mL, which was significantly lower than (5.05±3.04)×104/mL in the HC group (P < 0.000 1); the absolute number of γδT17 cells in the peripheral blood in SLE patients was (1.03±1.08)×103/mL, which was significantly higher than (0.40±0.24)×103/mL in the HC group (P < 0.05). The proportion of γδT17 cells in the peripheral blood of SLE patients was positively correlated with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) (r=0.59, P < 0.01) and erythrocyte sedimentation rate (r=0.65, P < 0.01), while the absolute number of γδT17 cells was positively correlated with the SLEDAI score (r=0.59, P < 0.01) and negatively correlated with the complement C3 level (r=-0.53, P < 0.05).
    Conclusion The significant decrease in the number of γδT cells in the peripheral blood of SLE patients is mainly due to the reduction of its γδT1 cell subset; γδT17 cells play an important role in the pathogenesis and activity of SLE.

     

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