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刺五加精制多糖对尼古丁诱导的学习记忆障碍小鼠的影响

Effects of acanthopanax refined polysaccharide in mice with nicotine-induced learning and memory impairment

    摘要
  • 摘要:
    目的 观察刺五加精制多糖（ASPS）对尼古丁诱导的学习记忆障碍小鼠的影响。
    方法 选取6周龄小鼠96只，雌雄各半，将其分为2批进行动物试验，即水迷宫试验和新物体识别试验。每批按小鼠体质量随机分为6组：空白组、模型组、阳性药物组、ASPS高剂量组、ASPS中剂量组、ASPS低剂量组。除空白组外，其余5组连续7 d每天皮下注射尼古丁0.5 mg/kg，制备尼古丁记忆障碍模型。第7天注射尼古丁24 h后，阳性药物组灌胃吡拉西坦800 mg/kg，ASPS高剂量组、ASPS中剂量组、ASPS低剂量组连续7 d分别灌胃ASPS 270、90、30 mg/kg。通过水迷宫试验和新物体识别试验分别检测小鼠的学习记忆能力。测试结束后，检测血清中超氧化物歧化酶（SOD）活性和海马组织中五羟色胺（5-HT）含量。
    结果 新物体识别试验结果显示，ASPS高、中、低剂量组辨别指数高于模型组，差异有统计学意义（P < 0.01或P < 0.05）。水迷宫试验结果显示，与模型组比较，ASPS高、中剂量组小鼠空间搜索试验中找到平台的时间缩短，差异有统计学意义（P < 0.05）；定位航行试验中，ASPS高剂量组小鼠平台进入次数多于模型组，差异有统计学意义（P < 0.05）；ASPS高、中剂量组小鼠Ⅲ象限路程比例高于模型组，差异有统计学意义（P < 0.05）。ASPS高、中、低剂量组SOD活性高于模型组，差异有统计学意义（P < 0.01）；小鼠海马组织含量测定显示，ASPS高、中剂量组小鼠5-HT含量高于模型组，差异有统计学意义（P < 0.01或P < 0.05）。
    结论 ASPS能显著提高尼古丁戒断小鼠的学习记忆能力，减轻海马神经递质的损伤，调节体内氧化应激能力，其机制可能与提高机体抗氧化能力、调节海马神经递质水平有关。

     

    Abstract:
    Objective To observe the effect of acanthopanax refined polysaccharide (ASPS) on nicotine-induced learning and memory impairment in mice.
    Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments: the Morris and the new object recognition batch. Each batch was randomly divided into 6 groups according to body weight: blank control group, model group, drug positive group, high-dose ASPS group, medium-dose ASPS group, and low-dose ASPS group. Except for the blank group, the remaining 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model. After 24 hours of injection of nicotine at the 7th day, the drug positive group was gavaged piracetam for 800 mg/kg, and the high-dose, medium-dose and low-dose ASPS groups were gavaged for 270, 90 and 30 mg/kg of ASPS respectively for 7 days. The learning and memory ability of mice was detected by water maze test and new object recognition test, respectively. After the two tests, superoxide dismutase(SOD) activity in serum and 5-Hydroxytryptamine (5-HT) content in hippocampal tissue were detected.
    Results The results of new object recognition experiment showed that the discrimination indexes of the high-, medium- and low-dose ASPS groups were significantly higher than that of the model group (P < 0.01 or P < 0.05). The results of water maze experiment showed that the time to find the platform in the spatial search experiment was significantly shorter in the high- and medium-dose ASPS groups than that in the model group (P < 0.05). In the positioning voyage test, the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group (P < 0.05); the proportions of Ⅲ quadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group (P < 0.05). The high-, medium- and low-dose ASPS groups were significantly higher than those in the model group (P < 0.01); the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high- and medium-dose ASPS groups was significantly higher than that in the model group (P < 0.01 or P < 0.05).
    Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice, relieve the damage of hippocampal neurotransmitters, and regulate oxidative stress in vivo. The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.

     

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