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长链非编码RNA X染色体失活特异转录物和微小RNA-146-5p在甲状腺癌中的表达及临床意义

Expression and clinical significance of long non-coding RNA X-inactive specific transcript and microRNA-146-5p in thyroid carcinoma

    摘要
  • 摘要:
    目的 探讨甲状腺癌组织中长链非编码RNA(LncRNA)X染色体失活特异转录物(XIST)、微小RNA-146-5p(miR-146-5p)的表达及其诊断价值。
    方法 选取甲状腺癌患者92例为研究对象，均行手术切除术，术中收集甲状腺癌组织和癌旁正常组织。分析LncRNA XIST、miR-146-5p水平与患者临床病理特征之间的关系。采用受试者工作特征(ROC)曲线评估LncRNA XIST、miR-146-5p单独及联合对甲状腺癌的诊断价值。
    结果 甲状腺癌组织中LncRNA XIST表达水平为(0.65±0.17), 低于癌旁组织的(1.02±0.18), miR-146-5p表达水平为(2.56±0.87), 高于癌旁组织的(1.07±0.25), 差异有统计学意义(P＜0.05)。临床分期Ⅲ~Ⅳ期、发生颈部淋巴结转移的甲状腺癌患者肿瘤组织中LncRNA XIST表达水平低于临床分期Ⅰ~Ⅱ期、未发生颈部淋巴结转移的甲状腺癌患者, miR-146-5p表达水平高于临床分期Ⅰ~Ⅱ期、未发生颈部淋巴结转移的甲状腺癌患者，差异有统计学意义(P＜0.05)。ROC曲线结果显示, LncRNA XIST、miR-146-5p二者联合诊断甲状腺癌的曲线下面积(AUC)大于LncRNA XIST、miR-146-5p单独诊断，差异有统计学意义(P＜0.05)。
    结论 甲状腺癌组织中LncRNA XIST表达下调, miR-146-5p表达上调，二者与临床分期、颈部淋巴结转移有关，并对甲状腺癌有一定的诊断价值。

     

    Abstract:
    Objective To explore the expression and diagnostic value of long non-coding RNA (LncRNA) X-inactive specific transcript (XIST) and microRNA-146-5p (miR-146-5p) in thyroid cancer tissue.
    Methods A total of 92 patients with thyroid cancer were selected as research objects. All patients underwent surgical resection, and thyroid cancer tissues and adjacent normal tissues were collected during the operation. The relationships between the levels of LncRNA XIST, miR-146-5p and the clinical pathological characteristics of patients were analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of LncRNA XIST and miR-146-5p alone and their combination in thyroid cancer.
    Results The expression level of LncRNA XIST in the thyroid cancer tissue was (0.65±0.17), which was significantly lower than (1.02±0.18) in the adjacent tissues, and the expression level of miR-146-5p was (2.56±0.87), which was significantly higher than (1.07±0.25) in the adjacent tissues (P < 0.05). The expression levels of LncRNA XIST in thyroid cancer patients with clinical stage Ⅲ to Ⅳ and cervical lymph node metastasis were significantly lower than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis, and the expression level of miR-146-5p was significantly higher than those in thyroid cancer patients with clinical stage Ⅰ to Ⅱ and no cervical lymph node metastasis (P < 0.05). ROC curve showed that the area under curve (AUC) of LncRNA XIST combined with miR-146-5p in was significantly higher than that of LncRNA XIST and miR-146-5p alone (P < 0.05).
    Conclusion The expression of LncRNA XIST is down-regulated and miR-146-5p is up-regulated in thyroid cancer. Both of them are related to clinical stage and cervical lymph node metastasis, and have certain diagnostic value for thyroid cancer.

     

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