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健脾祛风汤对特应性皮炎患者肠道菌群的影响

Effect of Jianpi Qufeng Decoction on intestinal flora in patients with atopic dermatitis

    摘要
  • 摘要:
    目的 探讨健脾祛风汤对特应性皮炎(AD)患者肠道菌群的影响。
    方法 选取30例AD儿童患者为治疗组(治疗前简称TB组，治疗后简称TA组)，予口服健脾祛风汤合并外涂尿素乳膏，治疗6周; 选取27例健康儿童作为健康对照组(N组)。治疗组于治疗前后采集粪便样品, N组无干预直接采集粪便样品。治疗前后进行SCORAD评分并进行临床疗效评价; 应用Illumina Miseq测序平台对研究对象粪便细菌的16SrRNA基因V3~V4区进行高通量测序; 采用Wilcoxon秩和检验进行物种丰度差异分析。
    结果 健脾祛风汤治疗AD的总有效率为96.67%, 治疗后SCORAD评分降低，差异有统计学意义(P < 0.001)。肠道菌群分析显示，在门水平，治疗后厚壁菌门(Firmicutes)丰度呈升高趋势，拟杆菌门(Bacteroidetes)丰度呈下降趋势，但差异无统计学意义(P>0.05)。在属水平，厚壁菌门下Blautia丰度呈升高趋势，差异有统计学意义(P < 0.05); 拟杆菌门下Bacteroides丰度呈现降低趋势，但差异无统计学意义(P>0.05)。在种水平，厚壁菌门下unclassified_g_Faecalibacterium、unclassified_g_ Eubacterium_rectale_group、Ruminococcus_sp. _5_1_39BFAA丰度呈现升高趋势，拟杆菌门下Bacteroides_vulgatus_ATCC_8482、Bacteroides_Xylanisolvens_g_Bacteroides、Bacteroides_uniformis丰度呈现降低趋势，但差异均无统计学意义(P>0.05)。
    结论 健脾祛风汤对AD的治疗作用可能与厚壁菌门及拟杆菌门物种丰度的变化有关。

     

    Abstract:
    Objective To investigate the effect of Jianpi Qufeng Decoction on intestinal flora in patients with atopic dermatitis (AD).
    Methods A total of 30 AD children were selected as treatment group (abbreviated as TB group before treatment, TA group after treatment). They were orally taken Jianpi Qufeng Decoction combined with external application of urea cream for 6 weeks. Twenty-seven healthy children were selected as healthy control group (N group). Fecal samples were collected before and after treatment in the treatment group and in the N group without intervention. SCORAD score and clinical efficacy were evaluated before and after treatment; Illumina Miseq sequencing platform was used to perform high-throughput sequencing of V3 to V4 region of 16SrRNA gene of fecal bacteria; Wilcoxon rank sum test was used to analyze differences in species abundance.
    Results The total effective rate of Jianpi Qufeng Decoction in the treatment of AD was 96.67%, and the SCORAD score was significantly decreased after treatment (P < 0.001). Intestinal microbiota analysis showed that at the phylum level, the abundance of Firmicutes increased and the abundance of Bacteroidetes decreased after treatment, but the difference was not statistically significant (P>0.05). At the genus level, the abundance of Blautia showed an increasing trend (P < 0.05); the abundance of Bacteroides showed a decreasing trend, but the difference was not statistically significant (P>0.05). At species level, under the phylum of Firmicutes, the abundance of unclassified_g_Faecalibacterium, unclassified_g_Eubacterium_rectale_group, Ruminococcus_sp._5_1_39BFAA showedincreasing trend, under the phylum of Bacteroidetes, the abundance of Bacteroides_vulgatus_ATCC_8482, Bacteroides_Xylanisolvens_g_Bacteroides, Bacteroides_uniformis showed decreasing trend, but the differences were not statistically significant (P>0.05).
    Conclusion The effect of Jianpi Qufeng Decoction in treating AD may be related to the changes in the abundance of Firmicutes and Bacteroidetes.

     

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