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具有CCCH锌指结构域的蛋白质12D在肺腺癌中的表达、预后及免疫特征分析

Expression, prognosis and immune characteristics of zinc finger CCCH domain-containing protein 12D in lung adenocarcinoma

    摘要
  • 摘要:
    目的  分析具有CCCH锌指结构域的蛋白质12D(ZC3H12D)在肺腺癌中的表达及预后价值, 探讨ZC3H12D的表达与肿瘤免疫细胞浸润的关系。
    方法 检索癌症基因组图谱(TCGA)数据库肺腺癌中ZC3H12D的表达情况，探讨临床病理特征与ZC3H12D表达的关系; 采用Kaplan-Meier方法和基于总生存期(OS)的单因素、多因素回归分析来评估ZC3H12D在肺腺癌中的预后价值; 利用GeneMANIA数据库分析肺腺癌中与ZC3H12D相关的蛋白质互作网络及信号通路; 利用TIMER、TISIDB数据库和ssGSEA方法分析ZC3H12D表达与肺腺癌中免疫细胞浸润水平的相关性。
    结果 肺腺癌中ZC3H12D的表达高于临近的正常组织，并与肺腺癌患者的临床病理特征相关，差异有统计学意义(P < 0.05), 表明高表达多预示预后不良。蛋白质互作网络分析发现, ZC2H12A、ZC2H12B、N4BP1、ZC2H12C、KHNYN、RPGRIP1L、NYNRIN、OR2V2、GPR75、ZNF488、FCER2、ZNF280B、GRAP、P2RX5、SLAMF6、CD80、TCF7、AICDA、PARP15和TUBB1等与ZC3H12D具有相互作用，且主要与免疫细胞信号通路相关。ZC3H12D表达与肿瘤浸润性免疫细胞呈正相关(P < 0.05)。
    结论 ZC3H12D是在肺腺癌中高表达的独立预后指标，且能影响免疫细胞浸润水平，或可作为肺腺癌潜在的诊断及预后生物标志物。

     

    Abstract:
    Objective To analyze the expression and prognostic value of zinc finger CCCH domain-containing protein 12D (ZC3H12D) in lung adenocarcinoma and explore the relationship of ZC3H12D expression with tumor immune cell infiltration.
    Methods The expression of ZC3H12D in lung adenocarcinoma was retrieved from The Cancer Genome Atlas (TCGA) database, and the relationship between clinicopathological features and expression of ZC3H12D was discussed; the Kaplan-Meier method and univariate and multivariate regression analyses based on overall survival (OS) were used to assess the prognostic value of ZC3H12D in lung adenocarcinoma; the ZC3H12D-related protein-protein interaction network and signaling pathway were analyzed by using the GeneMANIA database; TIMER, TISIDB database and ssGSEA method were used to analyze the correlation between ZC3H12D expression and the level of immune cell infiltration in lung adenocarcinoma.
    Results The expression of ZC3H12D in lung adenocarcinoma was significantly higher than that in adjacent normal tissues, and was significantly correlated with the clinicopathological features of patients with lung adenocarcinoma (P < 0.05), which indicated that high expression was more likely to predict poor prognosis. Protein-protein interaction network analysis revealed that ZC2H12A, ZC2H12B, N4BP1, ZC2H12C, KHNYN, RPGRIP1L, NYNRIN, OR2V2, GPR75, ZNF488, FCER2, ZNF280B, GRAP, P2RX5, SLAMF6, CD80, TCF7, AICDA, PARP15 and TUBB1 had significant interactions with ZC3H12D, and were mainly related to immune cell signaling pathways. The expression of ZC3H12D was positively correlated with tumor infiltrating immune cells (P < 0.05).
    Conclusion ZC3H12D is an independent prognostic indicator with high expression in lung adenocarcinoma, which can affect the level of immune cell infiltration, and it may be used as a potential biomarker for diagnosis and prognosis of lung adenocarcinoma.

     

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