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非肌层浸润性膀胱癌微小RNA-138、微小RNA-143表达与患者预后的相关性分析

Correlations of microRNA-138 and microRNA-143 expression with prognosis in patients with non-muscle invasive bladder cancer

    摘要
  • 摘要:
      目的  探讨非肌层浸润性膀胱癌(NMIBC)组织中微小RNA-138(miR-138)、微小RNA-143(miR-143)表达情况与预后的相关性。
      方法  选取124例NMIBC患者作为研究对象，其中68例患者同时取癌组织与癌旁组织(距离病灶约3 cm)。采用实时逆转录聚合酶链反应(RT-PCR)法检测NMIBC组织、癌旁组织中miR-138、miR-143表达水平。术后随访36个月，分析NMIBC患者的预后情况，应用多元Cox回归模型分析预后的危险因素。采用Log-rank χ2检验比较miR-138、miR-143不同表达量患者的生存率，并采用Kappa检验分析NMIBC组织中miR-138与miR-143表达的一致性。
      结果  NMIBC组织中miR-138、miR-143表达量低于癌旁组织，差异有统计学意义(P < 0.05)。多元Cox回归分析显示，肿瘤T1期、肿瘤直径≥3 cm、多发肿瘤、高级别乳头状尿路上皮癌(UPC)是预后的危险因素(P < 0.05), 而miR-138高表达、miR-143高表达是预后的保护因素(P < 0.05)。miR-138、miR-143高表达患者的无瘤生存率与累积生存率均高于miR-138、miR-143低表达患者，差异有统计学意义(P < 0.01)。Kappa检验结果提示, NMIBC组织中miR-138表达与miR-143表达具有高度一致性(P < 0.01)。
      结论  NMIBC组织中miR-138、miR-143表达均下调，而miR-138、miR-143表达降低可增加NMIBC患者预后不良风险，降低无瘤生存率与累积生存率。

     

    Abstract:
      Objective  To investigate correlations of expressions of microRNA-138(miR-138) and microRNA-143 (miR-143) with prognosis in patients with non-muscle invasive bladder cancer (NMIBC).
      Methods  A total of 124 patients with NMIBC were included as research objects, and tumor tissue and adjacent tissue (about 3 cm away from the lesion) were taken from 68 patients simultaneously. The expression levels of miR-138 and miR-143 in cancer tissues and adjacent tissues were detected by real-time reverse transcription polymerase chain reaction (RT-PCR). NMIBC patients were followed up for 36 months after surgery. Multivariate Cox regression model was used to analyze the prognostic risk factors. Log-rank chi-square test was used to analyze the correlation of miR-138 and miR-143 expression with prognosis. Kappa test was used to analyze the consistency of miR-138 and miR-143 expression in NMIBC tissues.
      Results  The expressions of miR-138 and miR-143 in NMIBC were lower than those in adjacent tissues (P < 0.05). Cox multiple regression analysis showed that tumor in T1 stage, tumor diameter ≥ 3 cm, multiple tumors and high-grade papillary urothelial carcinoma (UPC) were prognostic risk factors, while the high expressions of miR-138 and miR-143 were prognostic protective factors (P < 0.05). The tumor free and cumulative survival rates in patients with high expression of miR-138 and miR-143 were higher than those with low expressions of miR-138 and miR-143 (P < 0.01). Kappa test showed that the expression of miR-138 in NMIBC was highly consistent with that of miR-143 (P < 0.01).
      Conclusion  The expressions of miR-138 and miR-143 in NMIBC are down regulated, while decreased expressions of miR-138 and miR-143 could increase the risk of poor prognosis and reduce the tumor-free and cumulative survival rate of NMIBC.

     

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