高级检索

芳香烃受体mRNA、白细胞介素22在2型糖尿病中的作用研究

Roles of aryl hydrocarbon receptor mRNA and interleukin-22 in type 2 diabetes mellitus

    摘要
  • 摘要:
    目的 探讨2型糖尿病(T2DM)患者外周血单个核细胞中芳香烃受体(AhR) mRNA表达以及血清白细胞介素22(IL-22)水平。
    方法 将49例糖尿病前期患者、48例T2DM患者和50例健康对照者分别纳入糖尿病前期组、T2DM组、对照组。采用逆转录聚合酶链式反应(RT-PCR)法测定外周血单个核细胞中AhR mRNA的表达; 采用酶联免疫吸附法(ELISA)检测各组血清IL-22水平。测定各组空腹血糖(FBG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、胆固醇(CHO)、甘油三酯(TG)、空腹胰岛素(FINS)、餐后血糖(PPG)、糖化血红蛋白(HbA1c)和超敏C反应蛋白(hs-CRP)。
    结果 糖尿病前期组、T2DM组FBG、TG、PPG、HbA1c、hs-CRP均高于对照组, T2DM组HDL-C低于对照组, 差异有统计学意义(P< 0.01); T2DM组FBG、TG、PPG、HbA1c高于糖尿病前期组, HDL-C低于糖尿病前期组，差异有统计学意义(P<0.05或P< 0.01)。糖尿病前期组FINS、血清IL-22高于对照组，差异有统计学意义(P< 0.01); T2DM组FINS、血清IL-22、AhR mRNA相对表达含量均高于对照组和糖尿病前期组，差异有统计学意义(P< 0.01)。Pearson相关性分析显示, AhR mRNA、IL-22与年龄、CHO、TG、LDL-C、体质量指数均无相关性(P>0.05), 与FBG、FINS、PPG、HbA1c、hs-CRP呈正相关(P< 0.05或P< 0.01)。
    结论 通过检测AhR mRNA、IL-22水平对T2DM患者进行病情评估和预后判断具有一定的临床意义, AhR mRNA、IL-22或可成为糖尿病治疗研究的新靶标。

     

    Abstract:
    Objective To investigate the expression of aryl hydrocarbon receptor (AhR) mRNA in peripheral blood mononuclear cells and serum level of interleukin-22 (IL-22) in patients with type 2 diabetes mellitus (T2DM).
    Methods A total of 49 patients with pre-diabetes mellitus, 48 patients with T2DM and 50 healthy controls were respectively included in pre-diabetes group, T2DM group and control group. The expression of AhR mRNA in peripheral blood mononuclear cells was determined by reverse transcription polymerase chain reaction (RT-PCR); serum IL-22 level was detected by enzyme-linked immunosorbent assay (ELISA) in each group. Fasting blood glucose (FBG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), cholesterol (CHO), triglyceride (TG), fasting insulin (FINS), postprandial glucose (PPG), glycosylatedhemoglobin (HbA1c) and hypersensitive C-reactive protein (hs-CRP) were measured in each group.
    Results Levels of FBG, TG, PPG, HbA1c and hs-CRP in the pre-diabetes group and the T2DM group were significantly higher than those in the control group, and HDL-C in the T2DM group was significantly lower than that in the control group (P<0.01) levels of FBG, TG, PPG and HbA1c in the T2DM group were significantly higher than those in the pre-diabetes group, while the HDL-C level was significantly lower than that in the pre-diabetes group (P<0.05 or P<0.01). FINS and serum IL-22 in the pre-diabetes group were significantly higher than those in the control group (P<0.01); the FINS, serum IL-22 and relative expression of AhR mRNA in the T2DM group were significantly higher than those in the control group and the pre-diabetes group (P<0.01). Pearson correlation analysis showed that AhR mRNA and IL-22 had no correlations with age, CHO, TG, LDL-C and body mass index (P>0.05), but were positively correlated with FBG, FINS, PPG, HbA1c and hs-CRP (P<0.05 or P<0.01).
    Conclusion Detection of AhR mRNA and IL-22 level have certain clinical significance in evaluating the disease condition and prognosis of T2DM patients, which indicate AhR mRNA and IL-22 may become new targets for the treatment of diabetes.

     

    • HTML全文
    • 参考文献(24)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回