高密度脂蛋白对缺血性脑卒中急性期患者外周血中免疫细胞分布的影响

张海岳; 关经纬; 王族; 武霄; 宋海庆; 孟然

doi:10.7619/jcmp.20212439

高密度脂蛋白对缺血性脑卒中急性期患者外周血中免疫细胞分布的影响

Influence of high-density lipoprotein on immune cells distribution in peripheral blood of patients with ischemic stroke at acute stage

摘要

摘要:
目的分析高密度脂蛋白(HDL)对缺血性脑卒中急性期患者外周血中免疫细胞分布的影响。
方法纳入42例就诊于首都医科大学宣武医院的急性缺血性脑卒中患者为研究对象，所有患者发病至就诊时间均≤7 d。入院后治疗前均采集血标本，检测外周血中HDL的表达水平，应用流式细胞仪检测外周血中免疫细胞的百分比。根据HDL表达水平的不同将患者分为HDL < 1.0 mmol/L组(n=22)和HDL≥1.0 mmol/L组(n=20)。
结果 HDL < 1.0 mmol/L组外周血中CD3⁺T淋巴细胞、CD3⁺CD4⁺T淋巴细胞百分比高于HDL≥1.0 mmol/L组，但CD3⁺CD8⁺T淋巴细胞百分比低于HDL≥1.0 mmol/L组，差异均有统计学意义(P < 0.05)。2组患者外周血中单核细胞百分比差异无统计学意义(P > 0.05)。
结论在缺血性脑卒中急性期患者中，较高水平的HDL常伴有低水平的CD3⁺、CD3⁺CD4⁺T淋巴细胞的募集，这种结果可能有利于减少缺血性卒中后的脑组织损伤。

Abstract:
Objective To analyze the influence of high-density lipoprotein (HDL) on immune cells distribution in peripheral blood of patients with ischemic stroke at acute stage.
Methods Totally 42 patients with acute ischemic stroke in Xuanwu Hospital of Capital Medical University were selected as research objects, and the duration from onset to treatment was 7 days and below in all the patients. Blood samples were collected before treatment after admission, the expression level of HDL in peripheral blood was detected, and the percentage of immune cells in peripheral blood was detected by flow cytometer. The patients were divided into HDL < 1.0 mmol/L group (n=22) and HDL≥1.0 mmol/L group (n=20).
Results The percentages of CD3⁺ T lymphocytes and CD3⁺CD4⁺ T lymphocytes in peripheral blood in the HDL < 1.0 mmol/L group were significantly higher than those in the HDL≥1.0 mmol/L group, but the percentage of CD3⁺CD8⁺ T lymphocytes was significantly lower than that in the HDL≥1.0 mmol/L group (P < 0.05). There was no significant difference in the percentage of monocytes in peripheral blood between the two groups (P > 0.05).
Conclusion In patients with ischemic stroke at acute stage, the patients with higher level of HDL are often accompanied by the recruitment of low levels of CD3⁺ and CD3⁺CD4⁺ T lymphocytes, which may help to reduce brain tissue damage after ischemic stroke.

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