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辛伐他汀对慢性阻塞性肺疾病大鼠的抗炎作用及其机制研究

Study on anti-inflammatory action of simvastatin in rats with chronic obstructive pulmonary disease and its mechanism

    摘要
  • 摘要:
      目的    探讨辛伐他汀对慢性阻塞性肺疾病（COPD）大鼠的抗炎作用及其机制。
      方法    选择雄性SD大鼠50只，适应性饲养7 d，随机选择其中10只作为空白对照组，其余40只建立COPD模型，建模成功后随机分为4组模型组、低剂量组、中剂量组、高剂量组（使用辛伐他汀），每组10只。采集大鼠支气管肺泡灌洗液，检测炎性因子水平，检测维甲酸相关孤儿受体（ROR-γt）mRNA表达，并评估肺功能。
      结果    空白对照组大鼠肺功能指标水平最高，其余4组由高至低依次为高剂量组、中剂量组、低剂量组、模型组，其中低剂量组与中剂量组各肺功能指标水平比较，差异无统计学意义（P>0.05），其他组间比较，差异有统计学意义（P < 0.05）。空白对照组大鼠炎性因子各指标水平和ROR-γt相对表达最低，其余4组由低至高依次为高剂量组、中剂量组、低剂量组、模型组，其中低剂量与中剂量组炎性因子各指标水平、ROR-γt mRNA相对表达量比较，差异无统计学意义（P>0.05），其他组间炎性因子各指标水平、ROR-γt mRNA相对表达量比较，差异有统计学意义（P < 0.05）。
      结论    辛伐他汀对COPD大鼠有较理想的抗炎作用，其可能与药物抑制ROR-γt的表达有关，其机制可能为通过抑制炎症反应改善大鼠肺功能。

     

    Abstract:
      Objective     To study on anti-inflammatory action of simvastatin in rats with chronic obstructive pulmonary disease (COPD) and its mechanism.
      Methods     A total of 50 male SD rats were given 7 d adaptive feeding, and 10 rats were randomly selected and taken as blank control group, and the remaining 40 rats were used to establish COPD model. After successful modeling, they were randomly divided into four groupsthe model group, low-dose group, middle-dose group and high-dose group simvastatin, with 10 rats in each group. The bronchoalveolar lavage fluid of rats was collected, and the levels of inflammatory factors were detected. The expression of retinoic acid related orphan receptor ROR-γt mRNA was measured, and the pulmonary function was evaluated.
      Results     The levels of pulmonary function indexes of rats in blank control group were the highest, and the rank of the rest four groups from high to low was as follows: high-dose group, middle-dose group, low-dose group and model group. There were no statistically significant differences in the levels of inflammatory factors and the relative expression of ROR-γt mRNA between the low-dose and medium-dose groups (P>0.05). There were statistically significant differences in the levels of inflammatory factors and the relative expression of ROR-γt mRNA among other groups (P < 0.05).
      Conclusion     Simvastatin has an ideal anti-inflammatory effect for COPD rats, which may be related to the inhibition of ROR-γt expression by drugs, and the mechanism is that pulmonary function of rats can be improved by inhibiting inflammation.

     

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