Expression and clinicopathological significance of piRNA-19166 in esophageal squamous cell carcinoma
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摘要: 目的 检测食管鳞状细胞癌中piRNA-19166的表达水平,分析其临床病理意义。 方法 应用实时荧光定量聚合酶链式反应(qRT-PCR)技术检测piRNA-19166在96例食管鳞状细胞癌组织和癌旁正常组织中的表达,并分析其与食管鳞状细胞癌患者病理资料的关系。应用qRT-PCR检测piRNA-19166在3种食管癌细胞和正常食管上皮细胞中的表达。 结果 96例食管鳞状细胞癌组织中, 71例piRNA-19166表达下降, 25例表达正常或升高; 与癌旁正常食管组织相比, piRNA-19166在食管癌细胞株EC9706、TE-1、ECa10中均呈低表达,其中ECa10细胞、EC9706细胞中piRNA-19166低表达差异有统计学意义(P<0.05或P<0.01)。本研究结果显示, piRNA-19166低表达与分化程度、淋巴结转移、TNM分期有显著相关性(P<0.01)。 结论 PiRNA-19166可能是一种新的食管癌肿瘤抑制基因,低表达的piRNA-19166促进了食管癌的发生、发展和转移。
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关键词:
- piRNA-19166 /
- 食管鳞状细胞癌 /
- 实时荧光定量聚合酶链式反应
Abstract: Objective To detect the expression of piRNA-19166 in esophageal squamous cell carcinoma and analyze its clinicopathological significance. Methods The expression of piRNA-19166 was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)in tissues of esophageal squamous carcinoma and their paracancerous normal esophageal tissues of 96 cases, and the relationship between the expression of piRNA-19166 and pathological materials was analyzed. The qRT-PCR was used to detect the expressions of piRNA-19166 in three types of esophageal cancer cells and normal esophageal epithelial cells. Results In the 96 cases of esophageal squamous cell carcinoma, the expression of piRNA-19166 was decreased in 71 cases and was normal or increased in 25 cases. Compared with the paracancerous normal esophageal tissues, the expressions of piRNA-19166 in esophageal cancer cell lines EC9706, TE-1 and ECa10 were relatively low, and the expressions of piRNA-19166 in ECa10 and EC9706 cells were significantly lower than those in the paracancerous normal esophageal tissues(P<0.05 or P<0.01). The results showed that the low expression of piRNA-19166 was significantly correlated with the degree of differentiation, lymph node metastasis and TNM staging(P<0.01). Conclusion The piRNA-19166 may be a new tumor suppressor gene in esophageal cancer, and the low expression of piRNA-19166 promotes the occurrence, development and metastasis of esophageal cancer. -
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