Abstract: Objective To compare the effect of suture hemostasis and traditional electrocoagulation on ovarian function and pregnancy rate in patients with laparoscopic removal of benign ovarian tumors. Methods A total of 86 patients who underwent laparoscopic resection of benign ovarian tumors were selected, and were randomly divided into experimental group(n=43)and control group(n=43)according to different hemostasis methods. The experimental group was treated with suture hemostasis and the control group with traditional electrocoagulation hemostasis. The levels of follicle stimulating hormone(FSH), estradiol(E₂), luteinizing hormone(LH)before and at 3 months after surgery, the number of eggs, follicles and ovarian sinus follicles before and after operation were compared. The pregnancy conditions of the two groups were compared at 1 year after operation. Results There were no significant differences in preoperative FSH, E₂ and LH levels between the two groups(P>0.05); there was no significant difference in the LH level at 3 months after operation between the experimental group and the control group(P>0.05). The FSH level was significantly lower, and E₂ level was significantly higher than those of preoperation and the control group(P<0.05). There were no significant differences in the number of eggs, follicles and sinus follicles before - surgery between the two groups(P>0.05), and the above indicators after surgery in the two groups were significantly lower than operation before(P<0.05), but were significantly higher in the experimental group than the control group(P<0.05). The pregnancy rate was 69.77% in the experimental group and 62.79% in the control group, but no significant difference was found in two groups(P>0.05). Conclusion Laparoscopic removal of benign ovarian tumors by suture hemostasis has little effect on postoperative ovarian function and can be favorable for postoperative pregnancy.