Abstract: Objective To investigate the effects of pre-hospital early thrombolytic therapy on door-to-needle time(DNT)and neurological function in patients with acute ischemic stroke. Methods Totally 98 patients with acute ischemic stroke were divided into pre-hospital group(n=40)and intra-hospital group(n=58)according to application condition of recombinant tissue plasminogen activator(rt-PA)within 24 hours before hospital admission. The time to first medical contact(FMC), thrombolytic therapy time and recanalization rate were compared between the two groups. Results The thrombolytic therapy time of the pre-hospital group was significantly shorter than that of the intra-hospital group, and the recanalization rate was significantly higher than that of the intra-hospital group(P<0.05). Scores of National Institutes of Health Stroke Scale(NIHSS)in the pre-hospital group decreased at the time points of 2 h, 1 day and 7 days after treatment, and the NIHSS scores at the time points of 1 day and 7 days after treatment in the pre-hospital group were significantly lower than those in the intra-hospital group(P<0.05). Conclusion Application of pre-hospital thrombolysis in patients with acute ischemic stroke can significantly shorten DNT, enhance the effect of thrombolytic therapy, and improve the neurological function and ability of daily living.