Abstract: Objective To investigate the clinical features of small intestine intussusception and large intestine intussusception in children and evaluation of different intussusception by ultrasonography. Methods The data of 227 children with intussusception was retrospectively analyzed, with the number of 238 intussusception in total. According to clinical characteristics, ultrasound and air enema imaging findings, the patients were divided into small intestine intussusception group (112 intussusception) and large intestine intussusception group (116 intussusception). The clinical features and ultrasonographic features of the two groups were compared. Results The cases of blood in stool and abdominal mass of children with intestinal intussusception were significantly less than those of children with intestinal intussusception (P<0.01). The diameter and length of intussusception in small intestinal intussusception group were significantly shorter than those in large intestinal intussusception group (P<0.01). There was no significant difference in the number of lymph node enlargement between the two groups (P>0.05). The intussusception length of children with air enema was significantly longer, and the number of lymph nodes enlargement was significantly more than that of children with spontaneous remission (P<0.01). When the critical value of intussusception diameter was 2.15 cm, the Youden index was the largest and the screening effect of small intestine intussusception was the best, with the sensitivity of 83.6% and specificity of 92.6%. Conclusion Ultrasound plays an important role in the diagnosis of intestinal intussusception and large intestine intussusception.