Abstract: Objective To explore the effect of multidisciplinary health education in screening of retinopathy(ROP)in premature infants. Methods A total of 150 premature infants who were screened for retinopathy in our hospital were selected as research objects. They were divided into observation group and control group by random table method, with 75 cases in each group. The control group was given routine health education, and the observation group was given multidisciplinary health education, and the intervention effects were compared between the two groups. Results The cognitive score, visual acuity development score, eye infection prevention score and periodic examination score of the family members in the observation group were significantly higher than those of the control group(P<0.05). The re-visit rate, the severe rate, and the blindness rate of premature infants in the observation group were significantly lower than those in the control group(P<0.05). There was no significant difference in Clinical Activity Score(CAS)score between the two groups before treatment(P>0.05). After treatment, the CAS score of the observation group was(5.43±2.11), which was significantly lower than(7.43±2.21)of the control group(P<0.05). Conclusion Multidisciplinary health education can effectively improve the disease awareness of the family members of premature infants and their compliance behavior, effectively reduce the rate of re-visit of premature infants, and is conductive to reducing the rate of severe illness and blindness after accepting treatment of premature infants. Therefore, it has a better intervention effect.