Abstract: Objective To investigate the correlation between serum vitamin level, oxidative stress injury and preeclampsia in pregnant women. Methods A total of 46 pregnant women with preeclampsia were selected as study group, and another 50 normal pregnant women with healthy physical examination during the same period were selected as control group. The serum vitamin level, vascular endothelial injury index and oxidative stress injury index of pregnant women in the two groups were compared and analyzed. Results There was no significant difference in serum vitamin A level between the two groups(P>0.05); the serum levels of vitamin C and vitamin E in the study group were significantly lower than those in the control group(P<0.05); the levels of soluble endoglin(sEng)and endothelin 1(ET-1)in the study group were significantly higher than those in the control group, and the level of vascular endothelial growth factor(VEGF)was significantly lower than that in the control group(P<0.05); the levels of superoxide dismutase(SOD)and catalase(CAT)in the study group were significantly lower than those in the control group, and the level of reactive oxygen species(ROS)was significantly higher than those in the control group(P<0.05); the levels of matrix metalloproteinase-9(MMP-9), placental growth factor(PLGF)and total antioxidant capacity(T-AOC)in the study group were significantly lower than those in the control group(P<0.05). Conclusion Serum vitamin E and vitamin C are relatively deficient in preeclampsia pregnant women, and oxidative stress-mediated vascular endothelial function injury is involved in the development of preeclampsia, and these indicators can be taken as important predictors of preeclampsia.