Abstract: Objective To observe the application effect of green channel combined with emergency medical service in emergency treatment of acute ischemic stroke(AIS). Methods From May 2018 to May 2019, a total of 70 patients who received green channel combined with emergency medical service in emergency department were selected as observation group. A total of 70 patients who received routine emergency medical service in our hospital from April 2017 to May 2018 were selected as control group. The therapeutic effect, the treatment conditions of vascular intervention, intravenous thrombolysis and thrombolytic bridge endovascular intervention, and the time of thrombolysis and the National Institutes of Health Stroke Scale(NIHSS)score before and after treatment were compared between the two groups. Results The total effective rate in the observation group was significantly higher than that in the control group(94.29% vs. 78.57%, P<0.05). There were no significant differences in the treatment rates of vascular intervention and thrombolytic bridge intervention between the two groups(P>0.05); the effective rate of intravenous thrombolysis in the observation group was 22.86%, which was significantly higher than 10.00% in the control group(P<0.05). Thrombolysis time in the observation group was significantly shorter than that in the control group(P<0.05); before treatment, there was no significant difference in NIHSS scores between the two groups(P>0.05). After treatment, NIHSS score of the observation group was significantly lower than that of the control group(P<0.05). Conclusion Green channel combined with emergency medical services has a significant effect for AIS patients, which can optimize the diagnosis and treatment process, and shorten the time of thrombolysis and hospitalization.