Abstract: Objective To analyze the value of bedside ultrasonography in diagnosis of periventricular-intraventricular hemorrhage(PIVH)in premature infants. Methods The clinical data of 180 premature infants undergoing bedside ultrasonography was retrospectively analyzed. The continuous scanning in sagittal section, coronal section and transtemporal window cross-section was performed for all premature infants by bedside ultrasound. The intracranial lesions of all premature infants were observed, and the Papile Grading method was used to classify their degree of bleeding. A total of 10 premature infants without abnormalities firstly diagnosed by bedside ultrasound but were confirmed as PIVH after re-examination were included in control group, and another 10 premature infants with PIVH were selected as PIVH group, and cerebral arterial blood flow parameters［systolic peak velocity(V_s), diastolic peak velocity(V_d)and cerebral artery resistance index(RI)］ of two groups were compared. Results A total of 180 premature infants all underwent bedside ultrasonography, among whom 41 patients developed PIVH including 36(87.80%)cases with Papile Ⅰ grading, 3(7.32%)with Papile Ⅱ grading, and 2(4.88%)cases with Papile Ⅲ grading, with the total incidence of 22.78%. The V_s and V_d in the PIVH group were significantly lower, and RI was significantly higher than those in the control group(P<0.05). The incidence rates of PIVH in premature infants with gestational age ≥32 weeks(P=0.001)and in those with birth weight <1.5 kg(P=0.001)were significantly higher than those with gestational age<32 weeks and birth weight ≥1.5 kg. Conclusion Bedside ultrasound can - locate the bleeding sites in premature infants with PIVH, and monitor the changes of intracranial hemorrhage, which provides diagnostic value for PIVH in premature infants.