Abstract: Objective To investigate the effect of Yinzhihuang Oral Fluid combined with Bifid-triple Viable Powder on serum total bile acid, transferrin and C reactive protein(CRP)in neonates with jaundice. Methods A total of 500 jaundice neonates were randomly selected, and were divided into observation group and control group according to random number table method, with 250 cases in each group. The control group was given blue ray radiation therapy as well as oral administration of Yinzhihuang Oral Fluid, while the observation group was additionally given Bifid-triple Viable Powder. Serum total bile acid, transferrin, CRP, adverse reactions and growth as well as development of neonates in the two groups were compared. Results After treatment, the level of transferrin in the observation group was higher than that in the control group, and levels of CRP, bilirubin, total bile acid and incidence of adverse reactions were lower than those in the control group(P<0.05). After treatment, the body mass of neonates in the observation group was higher, and their body length and head circumference were better than those in the control group(P<0.05). Conclusion Yinzhihuang Oral Fluid combined with Bifid-triple Viable Powder can reduce levels of serum total bile acid, CRP, bilirubin and incidence of adverse reactions of jaundice neonates, improve the content of ferritin, and it is beneficial to the growth and development of children.