Abstract: Objective To investigate the effect of CD47 expression regulated by phosphatidylinositol 3-kinase(PI3K)/protein serine threonine kinase(AKT)pathway on the tumor invasiveness of glioblastoma. Methods Thirty samples of glioblastoma brain tissue confirmed by pathology were collected, and 10 normal samples of brain tissues were collected as controls. Effect of low and high expressions of CD47 on the invasiveness of glioblastoma were analyzed. Results CD47 was highly expressed in glioblastoma cells, and low expression of CD47 could significantly inhibit the tumor invasiveness, and high expression of CD47 could significantly promote the tumor invasiveness. CD47 was able to significantly enhance invasiveness of glioblastoma by activating the PI3K/AKT pathway(P<0.05). Conclusion CD47 is highly expressed in glioblastoma cells, and CD47 can significantly enhance invasiveness of glioblastoma by activating the PI3K/AKT pathway.