Abstract: In the past decades, nanoparticles have been widely used in energy generation, lubricant additives, chemical analysis and medical imaging. Meanwhile, scientists found that some nano materials have inherent enzyme simulation characteristics under physiological conditions, for example, iron oxide nanoparticles have high catalytic activity similar to natural enzymes(such as catalase or peroxidase). Therefore, they were named as nanozymes. In the past decade, these catalytic nanomaterials have been applied in some industrial and energy related field and has been as substitutes for natural enzymes. At first, nanozymes were mainly used in industrial field and in vitro detection, such as chemical synthesis, biomolecular detection, fuel processing or pollutant removal. In recent years, nanozymes has been used in medical field, including biofilm destruction, antioxidation, tissue regeneration, and prevention of tumor or anti-infection. In this paper, the application of nanozymes in biomedical science is reviewed, and its prospect in future is explored.