Abstract: Objective To investigate the effect of amoxicillin combined with omeprazole and metronidazole in treatment of patients with reflux esophagitis. Methods A total of 200 patients with reflux esophagitis were selected and randomly divided into two groups, with 100 cases in each group. The control group was treated with metoclopramide, while the study group was treated with amoxicillin, omeprazole and metronidazole. The efficacy was compared between two groups. Results After treatment, the total score of clinical symptoms in both groups was significantly lower than that before treatment, and that in the study group was significantly lower than the control group(P<0.05). After treatment, the total effective rate of symptom improvement in the study group was 97.00%, which was significantly higher than 88.00% in the control group(P<0.05). After treatment, the serum gastrin(GAS)of the two groups was significantly higher than that before treatment, and GAS of the study group was significantly higher than that of the control group(P<0.05). The nitric oxide(NO)after treatment in both groups was significantly lower than that before treatment, and the NO in the study group was significantly lower than that in the control group(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). Conclusion The combination of amoxicillin, omeprazole and metronidazole is effective and safe in the treatment of reflux esophagitis, which can regulate gastrointestinal hormones and reduce inflammatory reaction.