Abstract: Objective To investigate the therapeutic effect of Qufu Shengji Ointment combined with island-shaped stamp skin-grafting in patients with large area skin defect. Methods Thirty patients with large-scale skin contusion and skin degloving injury caused by violent trauma were selected, and had large skin necrosis and wound infection after debridement and suture in first stage. Patients were randomly divided into observation and control groups. The observation group used the Qufu Shengji Ointment for dressing change. After the granulation tissue covered the wound surface, the island-shaped stamp skin graft was carried out, and then the Qufu Shengji Ointment continued for dressing change. The control group underwent negative pressure closed drainage(VSD), and then routine debridement dressing was conducted, and then the conventional dressing continued. The skin preparation time, skin graft survival time, neonatal epithelial appearance time and wound healing time of the two groups were observed. Results After the skin grafting combined with the island stamp, the skin graft survival time and survival time of skin-grafting, growth rate of new epithelium and wound healing time were faster than the control group. At the time of follow-up, the wounds healed and the exposed areas of the bones and tendons were well covered. One patient had severe wound and healed slowly. After 3 months, the wound was finally healed. Conclusion Qufu Shengji Ointment can - promote granulation tissue growing, and shorten preparation time of skin grafting, improve the survival rate of skin graft and promote wound healing.